GAT1 Gene, the GATA Transcription Activator, Regulates the Production of Higher Alcohol during Wheat Beer Fermentation by Saccharomyces cerevisiae
Autor: | Dongguang Xiao, Ya-Ping Wang, Kun-Qiang Hong, Lin Liu, Xue-Shan Wang, Zhong-Guan Sun, Li-Hua Zhang |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
Technology QH301-705.5 Saccharomyces cerevisiae GATA factors Bioengineering Alcohol 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound Downregulation and upregulation 010608 biotechnology higher alcohol Biology (General) Ethanol metabolism Gene 030304 developmental biology 0303 health sciences biology Chemistry Free amino nitrogen Metabolism biology.organism_classification Biochemistry GAT1 Fermentation nitrogen catabolite repression |
Zdroj: | Bioengineering Volume 8 Issue 5 Bioengineering, Vol 8, Iss 61, p 61 (2021) |
ISSN: | 2306-5354 |
Popis: | Uncoordinated carbon-nitrogen ratio in raw materials will lead to excessive contents of higher alcohols in alcoholic beverages. The effect of GAT1 gene, the GATA transcription activator, on higher alcohol biosynthesis was investigated to clarify the mechanism of Saccharomyces cerevisiae regulating higher alcohol metabolism under high concentrations of free amino nitrogen (FAN). The availability of FAN by strain SDT1K with a GAT1 double-copy deletion was 28.31% lower than that of parent strain S17, and the yield of higher alcohols was 33.91% lower. The transcript levels of the downstream target genes of GAT1 and higher alcohol production in the double-copy deletion mutant suggested that a part of the effect of GAT1 deletion on higher alcohol production was the downregulation of GAP1, ARO9, and ARO10. This study shows that GATA factors can effectively regulate the metabolism of higher alcohols in S. cerevisiae and provides valuable insights into higher alcohol biosynthesis, showing great significance for the wheat beer industry. |
Databáze: | OpenAIRE |
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