Effects of Angelica gigas Nakai as an Anti-Inflammatory Agent in In Vitro and In Vivo Atopic Dermatitis Models

Autor: Deok-Seon Ryu, Sang-Ok Jeon, Sa-Rang Oh, Ji Wook Jung, Tae Sung Jung, Seon Ok
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Evidence-Based Complementary and Alternative Medicine, Vol 2018 (2018)
ISSN: 1741-4288
Popis: We investigated the cellular and molecular mechanisms mediating the effects ofAngelica gigasNakai extract (AGNE) through the mitogen-activated protein kinases (MAPKs)/NF-κB pathway usingin vitroandin vivoatopic dermatitis (AD) models. We examined the effects of AGNE on the expression of proinflammatory cytokines and chemokines in human mast cell line-1 (HMC-1) cells. Compound 48/80-induced pruritus and 2,4-dinitrochlorobenzene- (DNCB-) induced AD-like skin lesion mouse models were also used to investigate the antiallergic effects of AGNE. AGNE reduced histamine secretion, production of proinflammatory cytokines including interleukin- (IL-) 1β, IL-4, IL-6, IL-8, and IL-10, and expression of cyclooxygenase- (COX-) 2 in HMC-1 cells. Scratching behavior and DNCB-induced AD-like skin lesions were also attenuated by AGNE administration through the reduction of serum IgE, histamine, tumor necrosis factor-α(TNF-α), IL-6 levels, and COX-2 expression in skin tissue from mouse models. Furthermore, these inhibitory effects were mediated by the blockade of the MAPKs and NF-κB pathway. The findings of this study proved that AGNE improves the scratching behavior and atopy symptoms and reduces the activity of various atopy-related mediators in HMC-1 cells and mice model. These results suggest the AGNE has a therapeutic potential in anti-AD.
Databáze: OpenAIRE
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