CDKN1B/p27 expression in peripheral T cell lymphoma not otherwise specified
| Autor: | Pier Paolo Piccaluga, Claudia Mannu, Maria Teresa Sista, Maura Rossi, Francesco Bacci, Claudio Agostinelli, Anna Gazzola, Maria Rosaria Sapienza, Elena Sabattini, Pier Luigi Zinzani, Simona Righi, Stefano Pileri, Philip Went |
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| Přispěvatelé: | Gazzola A, Sista MT, Agostinelli C, Righi S, Sapienza MR, Mannu C, Rossi M, Bacci F, Sabattini E, Went P, Zinzani PL, Pileri SA, Piccaluga PP., Gazzola A., Sista M.T., Agostinelli C., Righi S., Sapienza M.R., Mannu C., Rossi M., Bacci F., SAbattini E., Went P., Zinzani P.L., Pileri S.A., Piccaluga P.P. |
| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Tumor suppressor gene T cell Gene Expression Peripheral T-cell lymphoma not otherwise specified Biology Polymorphism Single Nucleotide Pathology and Forensic Medicine CDKN1B/P27 T-Lymphocyte Subsets Gene expression medicine Humans RNA Messenger RNA Neoplasm Oligonucleotide Array Sequence Analysis Gene Expression Profiling PTCL NOS Intracellular Signaling Peptides and Proteins Lymphoma T-Cell Peripheral General Medicine Cell cycle Prognosis medicine.disease Survival Analysis Molecular biology Peripheral T-cell lymphoma Neoplasm Proteins Genes cdc medicine.anatomical_structure Regulatory sequence CDKN1B peripheral T-cell lymphomas Cyclin-Dependent Kinase Inhibitor p27 |
| Zdroj: | Journal of Clinical Pathology. 64:83-87 |
| ISSN: | 0021-9746 |
| Popis: | Aims Peripheral T cell lymphoma not otherwise specified (PTCL/NOS) is the commonest PTCL subtype. Recently, proliferation pathways have been found to be commonly altered in PTCL/NOS. CDKN1B/p27, a critical regulator of cell cycle and proliferation, has been suggested to be involved in T cell lymphomagenesis. This study aimed to evaluate the possible occurrence of CDKN1B/p27 aberrations in PTCL/NOS. Methods CDKN1B/p27 expression at RNA and protein level by DNA and tissue microarrays, in 28 and 98 cases, respectively, was studied. Additionally, direct sequencing of CDKN1B in 81 PTCL/NOS was performed. Results CDKN1B mRNA was similarly expressed in PTCL/NOS and normal T lymphocytes. In addition, structural abnormalities were not found; these included mutations and deletions in any exons, exon–intron junctions or regulatory regions. Furthermore, physiological expression of p27 in neoplastic cells was demonstrated by immunohistochemistry; this was mutually exclusive with Ki-67, as expected when the system is intact. Consistently, the expression of other molecules that are functionally related to CDKN1B/p27 in controlling cell cycle (including CCNE1) did not appear to be affected at either the mRNA or protein level. Finally, it was found that p27 expression was not significantly related with overall survival. Conclusion CDKN1B/p27 aberrations seem to be uncommon in PTCL/NOS pathogenesis. |
| Databáze: | OpenAIRE |
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