Increase in cytosolic and mitochondrial protein synthesis in rat hepatocytes irradiated in vitro by He-Ne laser
| Autor: | Rosa Anna Vacca, Salvatore Passarella, V.A. Petragallo, Margherita Greco, Ersilia Marra |
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| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Male
genetic structures Biophysics Stimulation Neon Cycloheximide Fluence Helium Photostimulation chemistry.chemical_compound Cytosol Protein biosynthesis Animals Radiology Nuclear Medicine and imaging Irradiation Rats Wistar Isolated hepatocytes Red light Cells Cultured Gel electrophoresis Radiation Radiological and Ultrasound Technology Lasers Molecular biology Mitochondria Rats chemistry Liver Protein Biosynthesis He-Ne laser Protein synthesis |
| Zdroj: | Journal of photochemistry and photobiology. B, Biology 34 (1996): 197–202. doi:10.1016/1011-1344(95)07280-2 info:cnr-pdr/source/autori:Vacca R.A.; Marra E.; Passarella S.; Petragallo V.A.; Greco M./titolo:Increase in cytosolic and mitochondrial protein synthesis in rat hepatocytes irradiated in vitro by He-Ne laser/doi:10.1016%2F1011-1344(95)07280-2/rivista:Journal of photochemistry and photobiology. B, Biology (Print)/anno:1996/pagina_da:197/pagina_a:202/intervallo_pagine:197–202/volume:34 |
| DOI: | 10.1016/1011-1344(95)07280-2 |
| Popis: | In order to gain an insight into the mechanism of cell photostimulation by laser light, protein synthesis was measured in hepatocytes irradiated with a low-power, continuous-wave HeNe laser (fluence, 0.24 J cm−2; fluence rate, 7 and 12 mW cm−2). As a result of irradiation the rate and amount of 35S-methionine incorporated into newly synthesized proteins increased, as demonstrated by gel electrophoresis and quantitative analysis of labelled protein bands. The stimulation of protein synthesis was fluence dependent, with a maximum stimulation at 0.24 J cm−2 for both fluence rates (12 and 7 mW cm−2). Both cytosolic and mitochondrial protein synthesis increased as a result of irradiation, as demonstrated by the measurement of hepatocytes previously treated with chloramphenicol and cycloheximide respectively. An initial investigation showed that stimulation of protein synthesis also occurred in hepatocytes irradiated with a non-coherent radiation source (fluence, 0.24 J cm−2). |
| Databáze: | OpenAIRE |
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