The Role of Marine Organic Extract in Bone Regeneration: A Pilot Study

Autor: Sharukh S. Khajotia, Rafaela Scariot, Fernando Luis Esteban Florez, Tatiana Miranda Deliberador, Mohammed Elsalanty, Ivana Vendramini, Leonardo Luiz Muller, Paola Fernanda Cotait de Lucas Corso, Viviane Rozeira Crivellaro, João César Zielak, Carmen Lucia Mueller Storrer
Rok vydání: 2019
Předmět:
Zdroj: BioMed Research International
BioMed Research International, Vol 2020 (2020)
ISSN: 2314-6141
Popis: Novel biomaterials capable of accelerating the healing process of skeletal tissues are urgently needed in dentistry. The present in vivo study assessed the osteoconductive and osteoinductive properties of experimental biphasic bioceramics (HA-TCP) modified or not by a nacre extract (marine organic extract, MOE) in a sheep model. Fabrication of MOE involved mixing ground nacre (0.05 g, particle sizes n = 8, age: 2 years, weight: 45 kg). Euthanization of animals was performed at 3 and 6 months after surgery. Tissues harvested were subjected to macroscopic and radiographic assessments. Specimens were then stained for histological analysis. Both control and experimental animals were capable of inducing the neoformation of fibrous connective tissue at both time points where superior amounts of tissue formation and mineralization were detected for experimental groups (unaltered (at 3 and 6 mos) and MOE-modified HA-TCP (at 3 mos)). Histological results, however, revealed that mature bone formation was only observed for specimens fabricated with MOE-modified HA-TCP in a time-dependent manner. The present study has successfully demonstrated the in vivo utility of experimental biphasic bioceramics modified by MOE in an ectopic grafting sheep model. Promising osteoconductive and osteoinductive properties must be further developed and confirmed by subsequent research.
Databáze: OpenAIRE
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