Induction of antibacterial activity in larvae of the blowflyLucilia sericataby an infected environment
| Autor: | Keiji Oguma, Takuya Kawabata, Kenji Yokota, Kozo Ishino, Hideya Mitsui, Shunji Sano |
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| Rok vydání: | 2010 |
| Předmět: |
Staphylococcus aureus
Time Factors animal structures medicine.medical_treatment Context (language use) Environment Biology medicine.disease_cause Lucilia Microbiology Maggot therapy medicine Animals Humans Calliphoridae music Ecology Evolution Behavior and Systematics music.instrument Debridement General Veterinary Maggot Diptera fungi biology.organism_classification Anti-Bacterial Agents Larva Insect Science Pseudomonas aeruginosa Wound Infection Parasitology Antibacterial activity |
| Zdroj: | Medical and Veterinary Entomology. 24:375-381 |
| ISSN: | 0269-283X |
| Popis: | Maggot debridement therapy (MDT) is a method for the treatment of intractable, infected and necrotic wounds. In MDT, sterile larvae of Lucilia sericata Meigen (Diptera: Calliphoridae) are applied to infected wounds, where they exert antibacterial effects. Once the larvae are placed in the wound, they are no longer germ-free. This study analysed the influence of infected environments on larval antibacterial activities. Sterile larvae were mixed in a test tube containing a bacterial suspension of Staphylococcus aureus or Pseudomonas aeruginosa, transferred to liver puree agar, and incubated at 25 °C for set periods. To collect the larval extracts, the incubated larvae were transferred to a test tube containing phosphate buffered saline (PBS), cut into multiple pieces with scissors, and centrifuged. The supernatant was used to test antibacterial activities. The results showed that infected larvae had better antibacterial capacities than sterile larvae. Antibacterial activities were induced by pretreatment with a single bacterial species, S. aureus or P. aeruginosa, within 24 h and 12 h, respectively, and disappeared after 36 h. The activities were effective against S. aureus, but not against P. aeruginosa. This natural infection model is very similar to the clinical wound context in MDT and will be a powerful tool with which to study the antibacterial activities of L. sericata larvae in MDT. |
| Databáze: | OpenAIRE |
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