Muscle LIM protein: expressed in slow muscle and induced in fast muscle by enhanced contractile activity
| Autor: | Achim G. Schneider, Dirk Pette, Karim R. Sultan |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Messenger ribonucleic acid
Male medicine.medical_specialty Transcription Genetic Physiology Total rna Molecular Sequence Data Muscle Proteins Stimulation Biology Antibodies Internal medicine Gene expression medicine Animals Amino Acid Sequence Cloning Molecular Rats Wistar Muscle Skeletal Transition (genetics) Myocardium Cell Biology Muscle LIM protein LIM Domain Proteins Peptide Fragments Recombinant Proteins Cell biology Rats Endocrinology Muscle Fibers Slow-Twitch Gene Expression Regulation Muscle Fibers Fast-Twitch Immunohistochemistry medicine.symptom Muscle contraction Muscle Contraction |
| Zdroj: | The American journal of physiology. 276(4) |
| ISSN: | 0002-9513 |
| Popis: | To identify early changes in gene expression during the fast-to-slow transition induced by chronic low-frequency stimulation, total RNA was extracted from 12-h-stimulated tibialis anterior (TA) muscles of rats and amplified by differential display RT-PCR. Among the signals of differentially expressed mRNAs, a cDNA ∼300 bp in length, which was almost undetectable in control TA muscles but prominent in stimulated TA and normal soleus muscles, was identified. This cDNA was cloned and identified as corresponding to the mRNA of the muscle LIM protein (MLP). Its differential expression in control, stimulated TA, and soleus muscles was verified by Northern blotting. Antibodies against MLP were used to identify by immunoblot analysis a protein of 22 kDa, the predicted molecular mass of MLP. Immunohistochemistry revealed strong reactivity for MLP in all fibers of normal soleus muscle and faint staining of some type IIA and type I fibers in control TA muscle. These fibers increased in number and staining intensity in 4-day-stimulated TA muscle. MLP thus seems to play an essential role during the rearrangement of cytoskeletal and/or myofibrillar structures in transforming adult muscle fibers. |
| Databáze: | OpenAIRE |
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