Oxygen concentration regulates 5-azacytidine-induced myogenesis in C3H/10T1/2 cultures
| Autor: | Thomas G. Storch |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cellular differentiation
Biology Methylation Cell Line chemistry.chemical_compound Cytosine Mice Myocyte Animals Molecular Biology Mice Inbred C3H Myogenesis Muscles Embryo Cytidine Cell Differentiation Cell Biology DNA Cell biology Oxygen Kinetics chemistry Biochemistry Cell culture 5-Methylcytosine Azacitidine Limiting oxygen concentration Stem cell |
| Zdroj: | Biochimica et biophysica acta. 1055(2) |
| ISSN: | 0006-3002 |
| Popis: | This study reports that changing the oxygen concentration within a physiologic range has a striking effect on myogenesis induced by the cytidine analog 5-azacytidine. Reducing oxygen from 20% to 2.5% increases 7-fold the number of myocytes that appear in cultures of C3H/10T1/2 mouse embryo cells 10 days after they receive a 24-h exposure to 5-azacytidine. Reducing oxygen does not alter the extent to which a 24-h exposure to 5-azacytidine inhibits cytosine methylation in newly synthesized DNA. Instead, the oxygen-sensitive step in myogenesis occurs after 5-azacytidine is removed from the culture medium. Reducing oxygen increases the rate of logarithmic growth in C3H/10T1/2 cultures after 5-azacytidine exposure, suggesting that survival and proliferation of myocyte stem cells (morphologically indistinguishable from uncommitted C3H/10T1/2 cells) may be the oxygen-sensitive steps in myogenesis. |
| Databáze: | OpenAIRE |
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