Irreversible binding of o,p′-DDD in interrenal cells of Atlantic cod (Gadus morhua)
| Autor: | Jørgen S. Christiansen, Ingvar Brandt, Örjan Lindhe, Kristian Ingebrigtsen |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Interrenal Gland
medicine.medical_specialty Environmental Engineering Health Toxicology and Mutagenesis Irreversible binding Administration Oral In Vitro Techniques Kidney Internal medicine medicine Animals Environmental Chemistry Gadus Carbon Radioisotopes Biotransformation Binding Sites biology Fishes Public Health Environmental and Occupational Health General Medicine General Chemistry equipment and supplies biology.organism_classification Pollution In vitro Endocrinology medicine.anatomical_structure Autoradiography Mitotane Atlantic cod Homeostasis Glucocorticoid medicine.drug |
| Zdroj: | Chemosphere. 50:1249-1253 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/s0045-6535(02)00550-7 |
| Popis: | Precision-cut tissue slices of the anterior kidney from Atlantic cod (Gadus morhua) were prepared with a Krumdieck tissue slicer and exposed to 2-(2-chlorophenyl)-2-(4-chloro-(14C)phenyl)-1,1-dichlorethane (o,p′-[14C]DDD) in vitro. Microautoradiography revealed irreversible o,p′-DDD-derived binding confined to the glucocorticoid producing interrenal cells (adrenocortical analogues). This cell-selective binding was confirmed by means of autoradiography at different levels of resolution on Atlantic cod administered o,p′-[14C]DDD intragastrically. The results provide evidence for a site-specific metabolic activation and irreversible binding of o,p′-DDD in the interrenal cells, which, in turn, may modify glucocorticoid homeostasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect