Degradation of extracellular matrix regulates osteoblast migration: A microfluidic-based study
| Autor: | Carlos Borau, José Manuel García-Aznar, Nieves Movilla, C. Valero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Histology Physiology Endocrinology Diabetes and Metabolism medicine.medical_treatment 0206 medical engineering Cell Culture Techniques 02 engineering and technology Matrix (biology) Matrix metalloproteinase Cell Line Extracellular matrix 03 medical and health sciences medicine Humans Bone regeneration Osteoblasts Chemistry Chemotaxis Growth factor Hydrogels Osteoblast Microfluidic Analytical Techniques 020601 biomedical engineering Extracellular Matrix 030104 developmental biology medicine.anatomical_structure Cell culture Self-healing hydrogels Biophysics |
| Zdroj: | Bone Zaguán. Repositorio Digital de la Universidad de Zaragoza instname |
| Popis: | Bone regeneration is strongly dependent on the capacity of cells to move in a 3D microenvironment, where a large cascade of signals is activated. To improve the understanding of this complex process and to advance in the knowledge of the role of each specific signal, it is fundamental to analyze the impact of each factor independently. Microfluidic-based cell culture is an appropriate technology to achieve this objective, because it allows recreating realistic 3D local microenvironments by taking into account the extracellular matrix, cells and chemical gradients in an independent or combined scenario. The main aim of this work is to analyze the impact of extracellular matrix properties and growth factor gradients on 3D osteoblast movement, as well as the role of cell matrix degradation. For that, we used collagen-based hydrogels, with and without crosslinkers, under different chemical gradients, and eventually inhibiting metalloproteinases to tweak matrix degradation. We found that osteoblast''s 3D migratory patterns were affected by the hydrogel properties and the PDGF-BB gradient, although the strongest regulatory factor was determined by the ability of cells to remodel the matrix. |
| Databáze: | OpenAIRE |
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