Gender- and age-related distinctions for the in vivo prooxidant state in Fanconi anaemia patients
| Autor: | Gerardo Beneduce, Marco d'Ischia, Anna Saviano, Frank J. Kelly, Adriana Zatterale, Paola Manini, Ana Lloret, Giovanni Pagano, Bruno Nobili, Federico V. Pallardó, Michel Warnau, Elena De Nicola, Sema Anak, Christina Dunster, Paolo Degan, Ebru E. Akisik, Rita Calzone, Emilia Vuttariello |
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| Přispěvatelé: | Pagano, G., Degan, P., D'Ischia, Marco, Kelly, F. J., Pallardo, F. V., Zatterale, A., Anak, S. S., Akisik, E. E., Beneduce, G., Calzone, R., DE NICOLA, E., Dunster, C., Lloret, A., Manini, Paola, Nobili, B., Saviano, A., Vuttariello, E., Warnau, M., Pagano, G, Degan, P, D'Ischia, M, Kelly, Fj, Pallardo, Fv, Zatterale, A, Anak, S, Akisik, Ee, Beneduce, G, Calzone, R, DE NICOLA, E, Dunster, C, Lloret, A, Manini, P, Nobili, Bruno, Saviano, A, Vuttariello, E |
| Rok vydání: | 2004 |
| Předmět: |
Male
Cancer Research medicine.medical_treatment Transplants Urine Ascorbic Acid medicine.disease_cause chemistry.chemical_compound Leukocytes Chromosomes Human Vitamin E Child Respiratory Burst Glutathione Disulfide Age Factors Chromosome Breakage General Medicine Pyruvaldehyde Glutathione Biochemistry 8-Hydroxy-2'-Deoxyguanosine Child Preschool Female Oxidation-Reduction Adult medicine.medical_specialty Heterozygote Adolescent Urinary system Biology Sex Factors Internal medicine medicine Humans Vitamin C Deoxyguanosine Infant DNA Ascorbic acid Uric Acid Oxidative Stress Endocrinology Fanconi Anemia chemistry Case-Control Studies Uric acid Reactive Oxygen Species Oxidative stress |
| Zdroj: | Carcinogenesis. 25(10) |
| ISSN: | 0143-3334 |
| Popis: | Some selected oxidative stress parameters were measured in 56 Fanconi anaemia (FA) patients (42 untransplanted and 14 transplanted), 54 FA heterozygotes (parents) and 173 controls. Untransplanted FA patients showed a highly significant increase in leukocyte 8-hydroxy-2’-deoxyguanosine (8-OHdG) (p = 0.00003) and a borderline increase (p = 0.076) in urinary levels of 8-OHdG vs. child controls. These increases were more pronounced in female FA patients (p = 0.00005 for leukocyte 8-OHdG, and p = 0.021 for urinary 8-OHdG). Female FA patients also displayed a highly significant excess of spontaneous chromosomal breaks vs. male patients (p = 0.00026), in the same female:male ratio (≅ 1.4) as detected both for leukocyte and for urine 8-OHdG levels. Plasma methylglyoxal (MGlx) levels were increased in untransplanted FA patients vs. child controls (p = 0.032). The increases in leukocyte and urinary 8-OHdG, and in MGlx levels were detected in young FA patients (≤15 yrs), whereas patients aged 16 to 29 yrs failed to display any differences vs. controls in the same age group. A significant increase in oxidised:reduced glutathione (GSSG:GSH) ratio was observed (p = 0.046) in the FA patients aged ≤15 yrs, whereas those aged 16 to 29 yrs, both untransplanted and transplanted, displayed a decrease (p = 0.06) in the GSSG:GSH ratio vs. the controls of the respective age groups. No significant changes were detected in plasma levels of Vitamin C, Vitamin E, or uric acid. Transplanted FA patients showed lesser alterations in leukocyte 8-OHdG and in GSSG:GSH ratio vs. untransplanted patients. The parents of FA patients displayed a significant increase in plasma MGlx levels (p = 0.0014) vs. adult controls. The results suggest a gender- and age-related modulation of oxidative stress in FA patients. The observed increase in urinary 8-OHdG in untransplanted FA patients suggests a proficient removal of oxidised DNA bases. Key words: Fanconi anaemia; 8-hydroxy-2’-deoxyguanosine; methylglyoxal; glutathione; chromosomal instability |
| Databáze: | OpenAIRE |
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