Activation/proliferation and apoptosis of bystander goat lymphocytes induced by a macrophage-tropic chimeric caprine arthritis encephalitis virus expressing SIV Nef
| Autor: | Stephanie Hoc-Villet, François Guiguen, Angela Rea, Opendra Narayan, Yahia Chebloune, Céline Garnier, Yuhuai Jin, Baya Amel Bouzar |
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| Přispěvatelé: | University of Kansas Medical Center [Lawrence], Rétrovirus et Pathologie Comparée (RPC), Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Arthritis-Encephalitis Virus
Caprine Genes Viral viruses [SDV]Life Sciences [q-bio] In Vitro Techniques medicine.disease_cause Recombinant virus Lymphocyte Activation Virus Replication Virus Article ACTIVATION 03 medical and health sciences Virology BYSTANDER medicine Animals Viral Regulatory and Accessory Proteins Lymphocytes Caprine arthritis encephalitis virus Tropism 030304 developmental biology Cell Proliferation DNA Primers 0303 health sciences NEF biology Base Sequence Chimera Goats Macrophages 030302 biochemistry & molecular biology virus diseases Simian immunodeficiency virus biology.organism_classification 3. Good health APOPTOSIS Viral replication Apoptosis Lentivirus DNA Viral Simian Immunodeficiency Virus |
| Zdroj: | Virology Virology, Elsevier, 2007, 364 (2), pp.269-280. ⟨10.1016/j.virol.2007.02.032⟩ |
| ISSN: | 0042-6822 1096-0341 |
| DOI: | 10.1016/j.virol.2007.02.032⟩ |
| Popis: | International audience; Caprine arthritis encephalitis virus (CAEV) is the natural lentivirus of goats, well known for its tropism for macrophages and its inability to cause infection in lymphocytes. The viral genome lacks nef, tat, vpu and vpx coding sequences. To test the hypothesis that when nef is expressed by the viral genome, the virus became toxic for lymphocytes during replication in macrophages, we inserted the SIVsmm PBj14 nef coding sequences into the genome of CAEV thereby generating CAEV-nef. This recombinant virus is not infectious for lymphocytes but is fully replication competent in goat macrophages in which it constitutively expresses the SIV Nef. We found that goat lymphocytes cocultured with CAEV-nef-infected macrophages became activated, showing increased expression of the interleukin-2 receptor (IL-2R). Activation correlated with increased proliferation of the cells. Interestingly, a dual effect in terms of apoptosis regulation was observed in exposed goat lymphocytes. Nef was found first to induce a protection of lymphocytes from apoptosis during the first few days following exposure to infected macrophages, but later it induced increased apoptosis in the activated lymphocytes. This new recombinant virus provides a model to study the functions of Nef in the context of infection of macrophages, but in absence of infection of T lymphocytes and brings new insights into the biological effects of Nef on lymphocytes. |
| Databáze: | OpenAIRE |
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