Characterization of Mycobacteria from a Major Brazilian Outbreak Suggests that Revision of the Taxonomic Status of Members of the Mycobacterium chelonae - M. abscessus Group Is Needed
| Autor: | Enrico Tortoli, Jesús Yubero, Maria Jesus Garcia, Sylvia Cardoso Leão, Cristina Viana-Niero, Maria Luiza Lopes, Suely Yoko Mizuka Ueki, Karla Valéria Batista Lima, María Carmen Menéndez |
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| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Genotype Mycobacterium Infections Nontuberculous Sequence Homology Mycobacterium chelonae Microbial Sensitivity Tests DNA Ribosomal Disease Outbreaks Mycobacterium Microbiology HaeIII Bacterial Proteins RNA Ribosomal 16S Mycobacterium bolletii medicine Cluster Analysis Humans Surgical Wound Infection Chromatography High Pressure Liquid Phylogeny Mycobacterium massiliense biology Phylogenetic tree Mycobacteriology and Aerobic Actinomycetes Sequence Analysis DNA bacterial infections and mycoses biology.organism_classification 16S ribosomal RNA rpoB Bacterial Typing Techniques Mycolic Acids bacteria Restriction fragment length polymorphism Brazil Polymorphism Restriction Fragment Length medicine.drug |
| Zdroj: | Journal of Clinical Microbiology. 47:2691-2698 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | An outbreak of postsurgical infections caused by rapidly growing mycobacteria has been ongoing in Brazil since 2004. The degrees of similarity of the rpoB and hsp65 sequences from the clinical isolates and the corresponding sequences from both the Mycobacterium massiliense and the M. bolletii type strains were above the accepted limit for interspecies variability, leading to conflicting identification results. Therefore, an extensive characterization of members of the M. chelonae-M. abscessus group was carried out. The M. abscessus , M. chelonae , M. immunogenum , M. massiliense , and M. bolletii type strains and a subset of clinical isolates were analyzed by biochemical tests, high-performance liquid chromatography, drug susceptibility testing, PCR-restriction enzyme analysis of hsp65 (PRA- hsp65 ), rpoB , and hsp65 gene sequencing and analysis of phylogenetic trees, DNA-DNA hybridization (DDH), and restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene (RFLP-16S rRNA). The clinical isolates and the M. abscessus , M. massiliense , and M. bolletii type strains could not be separated by phenotypic tests and were grouped in the phylogenetic trees obtained. The results of DDH also confirmed the >70% relatedness of the clinical isolates and the M. abscessus , M. massiliense , and M. bolletii type strains; and indistinguishable RFLP-16S rRNA patterns were obtained. On the contrary, the separation of clinical isolates and the M. abscessus , M. massiliense , and M. bolletii type strains from M. chelonae and M. immunogenum was supported by the results of PRA- hsp65 , DDH, and RFLP-16S rRNA and by the rpoB and hsp65 phylogenetic trees. Taken together, these results led to the proposition that M. abscessus , M. massiliense , and M. bolletii represent a single species, that of M. abscessus. Two subspecies are also proposed, M. abscessus subsp. abscessus and M. abscessus subsp. massiliense , and these two subspecies can be distinguished by two different PRA- hsp65 patterns, which differ by a single HaeIII band, and by differences in their rpoB (3.4%) and hsp 65 (1.3%) sequences. |
| Databáze: | OpenAIRE |
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