Gene expression profiles of rat olfactory bulb at developmental stage
| Autor: | Gi Jung Im, Jiwon Chang, Seo Jin Kim, Hak Hyun Jung, Jae Hoon Cho, Sang Ha K. Lee |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Candidate gene
Transcription Genetic Microarray Receptors Retinoic Acid medicine.drug_class Microarray analysis techniques Gene Expression Profiling Retinoic Acid Receptor alpha General Neuroscience Age Factors Gene Expression Regulation Developmental In situ hybridization Biology Olfactory Bulb Molecular biology Rats Olfactory bulb Animals Newborn Gene expression Basic Helix-Loop-Helix Transcription Factors medicine Animals Retinoid Gene Oligonucleotide Array Sequence Analysis |
| Zdroj: | Journal of Neuroscience Methods. 177:14-19 |
| ISSN: | 0165-0270 |
| DOI: | 10.1016/j.jneumeth.2008.09.012 |
| Popis: | Microarray analysis may be a useful tool to identify some candidate genes related to the development of olfactory bulbs. In the present study, gene expression profiles of olfactory bulbs from postnatal day 1 (P1) rats and postnatal day 35 (P35) rats were analyzed by oligonucleotide-microarray and expression levels of some selected genes were also confirmed by RT-PCR and in situ hybridization. 9146 genes were commonly identified in six microarray chips. Among these genes, 76 were up-regulated and 130 were down-regulated three-folds or more at P1 olfactory bulbs. Out of these 76 up-regulated genes, 24 genes were annotated based on the NCBI database of reference sequences and expression levels of these 24 genes were confirmed by RT-PCR. Among them, 2 interesting genes (neurogenic differentiation 1 and retinoid acid receptor alpha) were localized in the P1 olfactory bulb by the use of in situ hybridization technique. Our results may provide basic information to identify genes associated with functional growth of olfactory bulbs. |
| Databáze: | OpenAIRE |
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