The efficacy of HBOC-201 in ex situ gradual rewarming kidney perfusion in a rat model

Autor: Mohamed M. Aburawi, Paria Mahboub, Florence Lin, Shannon N. Tessier, Fermin Fontan, Murat Karabacak, James F. Markmann, Negin Karimian, Heidi Yeh, Korkut Uygun
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
0206 medical engineering
Biomedical Engineering
Medicine (miscellaneous)
Cold storage
Renal function
Hemodynamics
Bioengineering
DONOR LIVERS
02 engineering and technology
030204 cardiovascular system & hematology
Kidney
perfusion
Biomaterials
Hemoglobins
03 medical and health sciences
Oxygen Consumption
0302 clinical medicine
Blood Substitutes
Main Text Articles
Carbogen
medicine
Animals
Viaspan
PRESERVATION
Rewarming
Kidney transplantation
Main Text Article
business.industry
hemoglobin-based oxygen carrier
donor after circulatory death kidney graft
Equipment Design
Organ Preservation
General Medicine
medicine.disease
020601 biomedical engineering
Rats
3. Good health
medicine.anatomical_structure
Rats
Inbred Lew
Anesthesia
oxygen carrier
rewarming perfusion
hemoglobin‐based oxygen carrier
DONATION
NORMOTHERMIC MACHINE PERFUSION
business
Perfusion
Zdroj: ARTIFICIAL ORGANS. Wiley
Artificial Organs
ISSN: 0160-564X
Popis: Gradual rewarming from hypothermic to normothermic is a novel perfusion modality with superior outcome to sudden rewarming to normothermic. However, the identification of an oxygen carrier that could function at a temperature range from 4 to 7 degrees C or whether it is necessary to use oxygen carrier during kidney rewarming, remains unresolved. This study was designed to test the use of a hemoglobin-based oxygen carrier (HBOC) during gradual kidney rewarming as an alternative to simple dissolved oxygen. In this study, 10 rat kidneys were randomly divided into the control and the HBOC group. In the control group, no oxygen carrier was used during rewarming perfusion and the perfusion solution was oxygenated only by applying diffused carbogen flow. The protocol mimicked a donor after circulatory death (DCD) kidney transplantation, where after 30 minutes warm ischemia and 120 minutes cold storage in University of Wisconsin solution, the DCD kidneys underwent gradual rewarming from 10 to 37 degrees C during 90 minutes with or without HBOC. This was followed by 30 minutes of warm ischemia in room temperature to mimic the anastomosis time and 120 minutes of reperfusion at 37 degrees C to mimic the early post-transplant state of the graft. The HBOC group demonstrated superior kidney function which was highlighted by higher ultrafiltrate production, better glomerular filtration rate and improved sodium reabsorption. There was no significant difference between the 2 groups regarding the hemodynamics, tissue injury, and adenosine triphosphate levels. In conclusion, this study suggests better renal function recovery in DCD kidneys after rewarming with HBOC compared to rewarming without an oxygen carrier.
Databáze: OpenAIRE
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