Anti-inflammatory and proresolution activities of bergapten isolated from the roots of Ficus hirta in an in vivo zebrafish model
Autor: | Yi Yang, Jinhua Hu, Bangwei Yu, Deng Shanbin, Wenjie Mei, Yu Chuqin, Kangdi Zheng, Yandong Wang |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Male animal structures medicine.drug_class Neutrophils Biophysics Anti-Inflammatory Agents Inflammation Pharmacology Nitric Oxide Biochemistry Bergapten Plant Roots Anti-inflammatory Green fluorescent protein Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo medicine Animals Molecular Biology Zebrafish chemistry.chemical_classification Reactive oxygen species Wound Healing biology Chemistry Macrophages fungi Cell Biology biology.organism_classification Ficus Disease Models Animal 030104 developmental biology 030220 oncology & carcinogenesis 5-Methoxypsoralen Methoxsalen Female medicine.symptom Reactive Oxygen Species |
Zdroj: | Biochemical and biophysical research communications. 496(2) |
ISSN: | 1090-2104 |
Popis: | Bergapten (5-methoxypsoralen), a coumarin-derivate compound isolated from Ficus hirta roots, was evaluated for its anti-inflammatory and proresolution activities in a tail-cutting-induced zebrafish larvae model. Bergapten was evaluated using a caudal fin-wounded transgenic zebrafish line “Tg(corola: eGFP)” to visualize the effects of the recruitment and clearance of neutrophils and macrophages at the injury site. We found that bergapten significantly suppressed the recruitment of neutrophils and macrophages toward the injury site, as well as promoted the clearance of neutrophils and macrophages from the wound site. We also investigated the reactive oxygen species (ROS) and nitric oxide (NO) level of bergapten in a tail-cutting-induced inflammation zebrafish model. The Results revealed that bergapten effectively inhibited the tail-cutting-induced production of ROS and NO in zebrafish larvae. This study reported for the first time the potential anti-inflammatory and proresolution activities of bergapten in an in vivo zebrafish model, suggesting that bergapten may be a potential candidate for inflammation therapy. |
Databáze: | OpenAIRE |
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