A Dormant State Modulated by Osmotic Pressure Controls Clonogenicity of Prostate Cancer Cells
| Autor: | Maryline Havard, François Dautry, Thierry Tchénio |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
Osmosis medicine.medical_specialty Cell Biology Biochemistry Mice Cell Line Tumor Neoplasms Internal medicine medicine Animals Humans Osmotic pressure Neoplasm Metastasis Molecular Biology Cell Cycle Prostatic Neoplasms Cell Biology Cell cycle Activins Cell biology medicine.anatomical_structure Endocrinology Cell culture Culture Media Conditioned Cancer cell NIH 3T3 Cells Dormancy Tumor Suppressor Protein p53 Stem cell Signal transduction Signal Transduction |
| Zdroj: | Journal of Biological Chemistry. 286:44177-44186 |
| ISSN: | 0021-9258 |
| Popis: | Cell dormancy constitutes a limiting step of the metastatic process by preventing the proliferation of isolated cancer cells disseminated at distant sites from the primary tumor. The study of cancer cell dormancy is severely hampered by the lack of biological samples so that the mechanisms that regulate cell dormancy have not been extensively explored. In this work, we describe the rapid induction in vitro of a dormant state in prostate cancer cells by exposure to a slightly hypertonic growth medium. This quiescence is observed only when cells are seeded at low density and, once established, requires additional stimuli besides osmotic pressure to be reversed. Media conditioned by cells grown at high density can partially prevent or reverse dormancy, a phenomenon which can be reproduced with citric acid. In addition to this role of small metabolites, inactivation of the p53 and smad pathways also counters the entry into dormancy, whereas exposure to activin A induces it to some extent. Thus, this easily inducible dormancy reproduces several features associated with the dormancy of stem cells and cancer cells in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|