Sephadex® induced granulomatous reaction in rats
| Autor: | Brigitte Loenders, N. Buyssens, R. van den Bossche, A.G. Herman |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Pathology medicine.medical_specialty Vascular smooth muscle Injections Intradermal medicine.drug_class Toxicology Monoclonal antibody Drug Administration Schedule Pathology and Forensic Medicine Intubation Intratracheal medicine Animals Macrophage Rats Wistar Lung Granuloma biology business.industry Dextrans Arteries Cell Biology General Medicine Rats medicine.anatomical_structure Sephadex Giant cell Polyclonal antibodies Injections Intravenous biology.protein business Gels Blood vessel |
| Zdroj: | Experimental and toxicological pathology |
| ISSN: | 0940-2993 |
| Popis: | Summary Granuloma formation in the rat lung after single or repeated i.v. injections, intratracheal instillation and intradermal implantation of Sephadex® beads was studied over a time period from 3 h to 3 mo. Macrophages were identified with the mAB ED1, vascular smooth muscle cells with an mAB against α SMC-actin, endothelial cells with a polyclonal AB against factor VIII and cyclic activity with an mAB against PCNA. The morphology and the time course of the development of the granulomas is identical in the different experimental conditions. The macrophages form the bulk of the cellular infiltrates, giant cells appear after 24 h. Cyclic activity is early and marked in the interstitially located macrophages, it is delayed and slight around the beads, suggesting a biphasic pattern. The macrophage reaction is markedly enhanced after a second i.v. injection, indicating the development of a hypersensitivity state. Numerous eosinophils are located in the interstitium, but only few are in direct contact with the beads. Their number doesn't increase after a second i.v. injection. Intradermal implantations show only macrophages and lymphocytes. A special feature is the disappearance of the arterial wall around the beads resulting in extrusion without haemorrhages or thromboses. |
| Databáze: | OpenAIRE |
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