Rapid, practical and safe isolation of adipose derived stem cells
| Autor: | Ece Çerçi, Hatice Erdost |
|---|---|
| Přispěvatelé: | Bursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji-Embriyoloji Anabilim Dalı., Çerçi, Ece, Erdost, Hatice, AAH-9216-2021 |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell biology Histology Adipose tissue complex mixtures Isolation 03 medical and health sciences Laboratory flask 0302 clinical medicine Culture flasks Adipocytes Animals Cells Cultured Mesenchymal stem cell Cell Proliferation Confluency Tissue Adipocyte Stem cell Biotechnology & applied microbiology 030102 biochemistry & molecular biology Animal Chemistry Scratched flask Stem Cells Cell Differentiation Cell migration General Medicine equipment and supplies Rats Medical Laboratory Technology Adipose Tissue Adipogenesis Cell culture Differentiation 030220 oncology & carcinogenesis Extracellular-matrix Rat Stromal Cells Adipose Tissues Adipose Derived Stem Cell |
| Zdroj: | Biotechnic & Histochemistry. 96:138-145 |
| ISSN: | 1473-7760 1052-0295 |
| DOI: | 10.1080/10520295.2020.1776895 |
| Popis: | We compared migration, proliferation, growth curve, confluency and differentiation into adipogenic, osteogenic and chondrogenic cell lineages of mesenchymal stem cells derived from adipose tissue cultured in scratched and nonscratched cell culture flasks. Mesenchymal stem cells were isolated from rat adipose tissue using a nonenzymatic method. We investigated two groups. For the control group, minced adipose tissue was implanted conventionally onto the surface of standard plastic cell culture flasks. For the experimental group, the tissues were cultured in flasks with a scratched surface. We found that scratched flasks promoted cell migration, proliferation and confluency. Our findings suggest that scratched flasks may be used to ensure rapid, practical and safe isolation of adipose tissue-derived stem cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|