Effects of (3-amino-1-hydroxypropylidene)-1,1-bisphosphonate on mouse osteoclasts
| Autor: | Michael W. J. Davie, A. S. Wilson, Michael J. Marshall |
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| Rok vydání: | 2009 |
| Předmět: |
Calcitonin
musculoskeletal diseases medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Intraperitoneal injection Osteoclasts Pamidronate Parathyroid hormone Cell Count In Vitro Techniques Peptide hormone Mice Osteoclast Internal medicine Bone cell medicine Animals Orthopedics and Sports Medicine Carbon Radioisotopes Diphosphonates Chemistry Calcium Radioisotopes musculoskeletal neural and ocular physiology Bisphosphonate musculoskeletal system Endocrinology medicine.anatomical_structure Parathyroid Hormone Mice Inbred CBA cardiovascular system circulatory and respiratory physiology Hormone |
| Zdroj: | Journal of Bone and Mineral Research. 5:955-962 |
| ISSN: | 0884-0431 |
| DOI: | 10.1002/jbmr.5650050909 |
| Popis: | A group of 5-day-old mice were injected intraperitoneally with (3-amino-1-hydroxypropylidine)-1,1-bisphosphonate (APD). Morphologic changes were observed in vitally stained osteoclasts on parietal bones 3 days later, and these were judged to be degenerative. At this time significantly increased numbers of nuclei per osteoclast and total numbers of osteoclast nuclei were observed. However, at 4 days after the injection of APD, the total numbers of osteoclasts were significantly reduced relative to controls. When parietal bones were maintained in culture, APD reduced osteoclast numbers and inhibited cell-mediated 45Ca2+ release. Exposure of bones to parathyroid hormone increased the number of osteoclasts counted 1 day later. This effect was not blocked by APD. Calcitonin prevented the reduction in osteoclast numbers due to APD in vitro. We conclude that APD has a direct effect on resorbing mouse osteoclasts. |
| Databáze: | OpenAIRE |
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