Optical Stimulation of Thalamic Spindle Circuitry Sustains Electroencephalogram Patterns of General Anesthesia but not Duration of Loss of Consciousness
| Autor: | Lia Mesbah-Oskui, Richard L. Horner, Patrick Gurges, Wen-Ying Liu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Consciousness Thalamus Stimulation Sleep spindle Unconsciousness Electroencephalography Anesthesia General 03 medical and health sciences Mice 0302 clinical medicine Etomidate medicine Animals medicine.diagnostic_test business.industry General Neuroscience Mice Inbred C57BL 030104 developmental biology Anesthesia Anesthetic Reflex GABAergic business Sleep 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Neuroscience. 468 |
| ISSN: | 1873-7544 |
| Popis: | Alterations in thalamic GABAergic signaling are implicated in mediating the rise in 12-30 Hz electroencephalogram (EEG) activity that signals anesthetic-induced loss-of-consciousness with GABAA receptor-targeting general anesthetics. A number of modeling studies have identified that anesthetic-induced alterations in thalamocortico-corticothalamic signaling in the same network that generates sleep spindles would be sufficient to elicit this key EEG signature of anesthetic hypnosis with general anesthetic agents. Accordingly, we hypothesize that targeted stimulation of this thalamic GABAergic circuitry into a sleep-spindle mode of activity would promote the general anesthetic effects of etomidate. We recorded EEG activity and loss-of-righting reflex in transgenic mice expressing channel rhodopsin-2 on GABAergic neurons (ChR2-VGAT, n = 8) and control, wild-type mice (C57BL/6J, n = 8). On two consecutive days mice were randomly assigned to receive spindle-rhythm stimulation via an optical probe targeting the left reticular thalamic nucleus or no stimulation. After an initial 30-minute recording, mice were administered etomidate (12 mg/kg, intraperitoneal) and recorded for 90 min with or without optical stimulation. Etomidate elicited an increase in 12-30 Hz EEG power in wild-type and ChR2-VGAT mice for 20 min following administration (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect