MiR‐200b expression in breast cancer: a prognostic marker and act on cell proliferation and apoptosis by targeting Sp1
Autor: | Shihai Liu, Zan Shen, Xingang Wang, Wensheng Qiu, Lu Yue, Ruyong Yao, Yong Li, Jian Hu, Yasai Yao, Hui Cong |
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Rok vydání: | 2015 |
Předmět: |
Sp1 Transcription Factor
Blotting Western MiR-200b Apoptosis Breast Neoplasms Kaplan-Meier Estimate Biology Sp1 breast cancer Western blot Cell Line Tumor microRNA Biomarkers Tumor medicine cell growth Humans 3' Untranslated Regions Cell Proliferation Neoplasm Staging Gene knockdown medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Cell growth Cancer Original Articles Cell Biology Middle Aged Cell cycle Prognosis medicine.disease Molecular biology Gene Expression Regulation Neoplastic MicroRNAs Cell culture MCF-7 Cells Molecular Medicine Female RNA Interference |
Zdroj: | Journal of Cellular and Molecular Medicine |
ISSN: | 1582-4934 1582-1838 |
Popis: | MicroRNAs (miRNAs) have been identified as important post-transcriptional regulators involved in various biological and pathological processes of cells. In the present study, we investigated the roles and mechanisms of miR-200b in human breast cancer (BC). MiR-200b expression was carried out by qRT-PCR in human BC cell lines and clinical samples and the prognostic potential of miR-200b expression was further evaluated. In vitro, effects of miR-200b on BC cell proliferation, apoptosis and cell cycle distribution were tested by CCK-8 kit, flow cytometric analysis respectively. Luciferase assay and Western blot analysis were performed to validate the potential targets of miR-200b after the preliminary screening by employing open access software. We found that miR-200b was significantly down-regulated in both BC tissues and cell lines. The low expression of miR-200b was correlated with late TNM stage, negative oestrogen receptor and positive HER-2 status. Multivariate analysis showed that miR-200b expression was an independent prognostic predictor for BC patients. Integrated analysis identified Sp1 as a direct and functional target of miR-200b. Knockdown of Sp1 inhibited cell proliferation, induce apoptosis and act on cell cycle resembling that of miR-200b high expression. Our data demonstrates that miR-200b has potential to serve as prognostic biomarker and tumour suppressor for BC patients. As a direct and functional target of miR-200b, Sp1 and miR-200b both could be an exciting target for BC treatment strategy. |
Databáze: | OpenAIRE |
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