A Rapid and Highly Sensitive UPLC-MS-MS Method for the Quantification of Zolpidem Tartrate in Human EDTA Plasma and its Application to Pharmacokinetic Study
| Autor: | A. T. Bapuji, S. Ravinder, Vepakomma Suryanarayana Rao, Dendhi Chandrapal Reddy, Vurimindi Himabindu |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Detection limit Spectrometry Mass Electrospray Ionization Chromatography Pyridines Chemistry Electrospray ionization Zolpidem Tartrate Selected reaction monitoring Ethyl acetate General Medicine Tandem mass spectrometry Mass spectrometry Sensitivity and Specificity Analytical Chemistry Triple quadrupole mass spectrometer Zolpidem Young Adult chemistry.chemical_compound Tandem Mass Spectrometry Humans Chromatography High Pressure Liquid Edetic Acid |
| Zdroj: | Journal of Chromatographic Science. 50:538-546 |
| ISSN: | 1945-239X 0021-9665 |
| DOI: | 10.1093/chromsci/bms070 |
| Popis: | A rapid and high sensitive liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed and validated for the quantification of zolpidem in human EDTA plasma using ondansetron (IS) as an internal standard. The analyte and IS were extracted from human plasma using ethyl acetate and separated on a C18 column (Inertsil-ODS, 5 µm, 4.6 × 50 mm) interfaced with a triple quadrupole tandem mass spectrometer. The mobile phase, which consisted of a mixture of methanol and 20 mM ammonium formate (pH 5.00 ± 0.05; 75:25 v/v), was injected at a flow rate of 0.40 mL/min. The retention times of zolpidem and IS were approximately 1.76 and 1.22. The LC run time was 3 min. The electrospray ionization source was operated in positive ion mode. Multiple reaction monitoring used the [M + H](+) ions m/z 308.13 → 235.21 for zolpidem and m/z 294.02 → 170.09 for the ondansetron, respectively. Five freeze-thaw cycles was established at -20 and -70°C.The linearity of the response/concentration curve was established in human EDTA plasma over the concentration range 0.10-149.83 ng/mL. The lower detection limit [(signal-to-noise (S/N) > 3] was 0.04 ng/mL and the lower limit of quantification (S/N > 10) was 0.10 ng/mL. This LC-MS-MS method was validated with intra-batch and inter-batch precision of 0.52-8.66.The intra-batch and inter-batch accuracy was 96.66-106.11. Recovery of zolpidem in human plasma was 87.00% and IS recovery was 81.60%. The primary pharmacokinetic parameters were T(max) (h) = (1.25 ± 0.725), C(max) (ng/mL) (127.80 ± 34.081), AUC(0→t), = (665.37 ± 320.982) and AUC(0→∞), 686.03 ± 342.952, respectively. |
| Databáze: | OpenAIRE |
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