Enzymatic Digestion of Porcine Corneas Cross-linked by Hypo- and Hyperosmolar Formulations of Riboflavin/ultraviolet A or WST11/Near-Infrared Light
| Autor: | Jurriaan Brekelmans, Petra F. G. Wolffs, Arie L. Marcovich, Alexa Goz, Koen Rieff, Alexander Brandis, Tos T. J. M. Berendschot, Judith Veugen, Rudy M.M.A. Nuijts, Mor M. Dickman, Avigdor Scherz |
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| Přispěvatelé: | MUMC+: MA UECM AIOS (9), RS: MHeNs - R3 - Neuroscience, MUMC+: MA UECM Oogartsen MUMC (9), Oogheelkunde, RS: NUTRIM - R3 - Respiratory & Age-related Health, RS: CAPHRI - R4 - Health Inequities and Societal Participation, Med Microbiol, Infect Dis & Infect Prev, RS: NUTRIM - R1 - Obesity, diabetes and cardiovascular health, MUMC+: *AB Refractie Chirurgie Oogheelkunde (9), Medical Image Analysis |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Swine STROMA Biomedical Engineering Corneal collagen cross-linking Riboflavin Article Cornea ENERGY 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Suidae medicine Ultraviolet light Animals WST-D Photosensitizing Agents/pharmacology KERATOCONUS riboflavin Bacteriochlorophylls Chromatography Photosensitizing Agents biology Chemistry Riboflavin/pharmacology DEGRADATION biology.organism_classification Cross-Linking Reagents/pharmacology collagenase Ophthalmology 030104 developmental biology Dextran medicine.anatomical_structure Cross-Linking Reagents LINKING 030221 ophthalmology & optometry Collagenase ROSE-BENGAL Digestion Collagen keratolysis corneal collagen cross-linking RESISTANCE medicine.drug |
| Zdroj: | Translational Vision Science & Technology, 9(10):4. Association for Research in Vision and Ophthalmology Translational Vision Science & Technology Translational Vision Science & Technology, 9(10):4. Association for Research in Vision and Opthalmology |
| ISSN: | 2164-2591 |
| Popis: | Purpose: To assess enzymatic digestion rate after Riboflavin (RF) and Water-Soluble-Taurine (WST11) based corneal cross-linking (CXL), with or without the addition of high molecular weight dextran (RF-D and WST-D).Methods: Eighty-eight paired porcine corneas were cross-linked by either RF (n = 11) or RF-D (n = 11) and ultraviolet light (UVA), or WST11 (n = 11) or WST-D (n = 11) and near-infrared (NIR) light, or used as paired control (n = 44). Corneal buttons of treated and paired control eyes were placed in a 0.3% collagenase solution. Time to full digestion and remaining dry sample weight after six hours were compared.Results: A strong treatment effect was seen with all four formulations, as all controls had been fully digested whilst all treated samples were still visible at the experiment's endpoint. After irradiation, central corneal thickness was significantly higher in samples treated with hypo-osmolar formulations, compared to dextran enriched formulations (P < 0.001). Dry sample weight after digestion was nonsignificantly different between corneas treated by the four different formulations (P = 0.102). Average dry sample weight was 1.68 ± 0.6 (n = 10), 2.19 ± 0.50 (n = 8), 1.48 ± 0.76 (n = 11), and 1.54 ± 0.60 (n = 9) mg, for RF, RF-D, WST11, and WST-D treated samples, respectively. Enzymatic resistance was similar for RF and WST based CXL (P = 0.61) and was not affected by the addition of dextran (P = 0.221).Conclusions: Both RF and WST11 based CXL significantly increases resistance to enzymatic digestion, with similar effect for hypo-osmolar and hyperosmolar (dextran enriched) formulations.Translational Relevance: Our findings indicate these formulations are interchangeable, paving the way for the development of novel PACK-CXL protocols for thin corneas and deep-seated infections. |
| Databáze: | OpenAIRE |
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