Construction and identification of recombinant adenovirus vector containing the hVEGF165 gene
| Autor: | Yan Jin, Lu Zaiying, Liu Qigong, Zhang Weidong |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Vascular Endothelial Growth Factor A
endocrine system DNA Complementary Vascular smooth muscle Genetic enhancement Genetic Vectors Endothelial Growth Factors Biology Muscle Smooth Vascular Umbilical vein Adenoviridae law.invention Viral vector chemistry.chemical_compound Western blot law Management of Technology and Innovation Gene expression medicine Animals Humans Cells Cultured Recombination Genetic Lymphokines medicine.diagnostic_test Vascular Endothelial Growth Factors Molecular biology Vascular endothelial growth factor chemistry cardiovascular system Recombinant DNA Rabbits Cell Division Plasmids |
| Zdroj: | Journal of Tongji Medical University. 20:186-189 |
| ISSN: | 0257-716X |
| DOI: | 10.1007/bf02886984 |
| Popis: | To construct the recombinant adenovirus vector containing the cDNA for human vascular endothelial growth factor (hVEGF165), the cDNA for hVEGF165 was subcloned into pACCMV · pLpA. Subsequently, this recombinant pACCMV · hVEGF was co-transfected into 293 cells together with pJM17 to obtain the replication-deficient recombinant adenovirus containing hVEGF gene — Ad-CMV · hVEGF. The VEGF gene expression was detected by using RT-PCR and Western blot in rabbit aorta vascular smooth muscle cells (VSMC) infected with AdCMV · hVEGF. Cultured human umbilical vein endothelial cells (HUVEC) were incubated with the conditioned medium (CM) from above mentioned VSMC infected with AdCMV · hVEGF to observe the effect of VEGF on proliferation of HUVEC. 48 h after the infection with AdCMV · hVEGF, VSMC demonstrated VEGF expression, and the expressed VEGF could stimulate the proliferation of HUVECin vitro. Successfully prepared AdCMV · hVEGF165 could express biologically active VEGF in infected VSMC, and stimulate proliferation of HUVEC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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