DNA barcoding of freshwater fishes from the transboundary river of Indo-Bhutan: multiple clades and cryptic diversity
Autor: | Kailash Chandra, Laishram Kosygin, Vikas Kumar, Kaomud Tyagi, Shibananda Rath, Avas Pakrashi, Shantanu Kundu, Ujjal Das |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Species complex cryptic species media_common.quotation_subject conservation Species diversity Biology 010603 evolutionary biology 01 natural sciences DNA barcoding Mito Communication 03 medical and health sciences 030104 developmental biology Evolutionary biology Genetics biodiversity sharing Ichthyofauna Clade Molecular Biology Diversity (politics) media_common Research Article |
Zdroj: | Mitochondrial DNA. Part B, Resources article-version (VoR) Version of Record |
ISSN: | 2380-2359 |
Popis: | The species diversity of freshwater fishes from the transboundary river, Jaldhaka is still unknown to the scientific communities. We generated 40 DNA barcode sequences of 16 morphologically identified freshwater fishes and compared genetically with the database sequences. Ten species (Acanthocobitis botia, Barilius bendelisis, Crossocheilus latius, Channa punctata, Channa quinquefasciata, Garra gotyla, Garra kempi, Opsarius barna, Psilorhynchus balitora, and Pseudecheneis sulcata) showed unique haplotypes in the studied riverine system. Further, the estimated genetic divergences, BA tree topology, and ABGD species delimitation methods revealed the presence of cryptic diversity in Badis badis, Garra annandalei, G. gotyla, G. kempi, P. balitora, Rasbora daniconius, and Pethia ticto. The study suggested more exhaustive sampling and generation of more molecular data to strengthen the fact. The aimed integrated approach will be helpful to detect the extant species diversity, helps to reevaluate the checklist and promote sustainable conservation management to protect this unparalleled ecosystem. |
Databáze: | OpenAIRE |
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