Association of Retinoic Acid Receptors with Extracellular Matrix Accumulation in Rats with Renal Interstitial Fibrosis Disease
| Autor: | Tian-Biao Zhou, Feng-Ying Lei, Yuan-Han Qin, Yao-Bin Long |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Pathology Receptors Retinoic Acid renal interstitial fibrosis Retinoic acid Gene Expression lcsh:Chemistry chemistry.chemical_compound Fibrosis Gene expression Receptor lcsh:QH301-705.5 Spectroscopy RARs biology Retinoic Acid Receptor alpha General Medicine Computer Science Applications Reverse transcription polymerase chain reaction embryonic structures Kidney Diseases RARα Collagen Type IV medicine.medical_specialty extracellular matrix Article Catalysis Transforming Growth Factor beta1 Inorganic Chemistry retinoic acid receptors RARα RARβ RARγ Internal medicine medicine Animals Physical and Theoretical Chemistry neoplasms Molecular Biology Messenger RNA business.industry organic chemicals Organic Chemistry medicine.disease biological factors Fibronectins Rats body regions Fibronectin Disease Models Animal Endocrinology lcsh:Biology (General) lcsh:QD1-999 chemistry Retinoic acid receptor alpha biology.protein business |
| Zdroj: | International Journal of Molecular Sciences; Volume 13; Issue 11; Pages: 14073-14085 International Journal of Molecular Sciences, Vol 13, Iss 11, Pp 14073-14085 (2012) International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms131114073 |
| Popis: | The nuclear retinoic acid receptors (RARs) function as ligand-dependent transcriptional regulators and include three subtypes (RARα, RARβ and RARγ), which control the expression of specific gene subsets subsequent to ligand binding and to strictly controlled phosphorylation processes. Extracellular matrix (ECM) accumulation is the most important characteristic of renal interstitial fibrosis (RIF). This study was performed to investigate whether RARs were associated with ECM accumulation in the progression of RIF in rats. Eighty Wistar male rats were divided into a sham operation group (SHO) and a model group subjected to unilateral ureteral obstruction (GU) at random; n = 40, respectively. The RIF disease in GU group was established by left ureteral ligation. The renal tissues were collected at two weeks and four weeks after surgery. Protein expressions of RARα, RARβ, RARγ, transforming growth factor-βl (TGF-β1), collagen-IV (Col-IV) and fibronectin (FN) were detected using immunohistochemical analysis, and mRNA expressions of RARα, RARβ, RARγ and TGF-β1 in renal tissue were detected by real time reverse transcription polymerase chain reaction. RIF index in renal interstitium was also calculated. When compared with those in SHO group, expressions of RARα and RARβ (protein and mRNA) were markedly reduced in the GU group (each p < 0.01). There was no marked difference for the expression of RARγ (protein and mRNA) between the SHO group and the GU group. The expressions of TGF-β1, Col-IV, FN and the RIF index in the GU group were markedly increased when compared with those in the SHO group (each p < 0.01). The protein expression of RARα/RARβ was negatively correlated with protein expression of TGF-β1, Col-IV or FN and the RIF index (all p < 0.01). In conclusion, the low expression of RARα/RARβ is associated with ECM accumulation in the progression of RIF in rats, suggesting that RARα/RARβ is a potentially therapeutic target for prevention of RIF. |
| Databáze: | OpenAIRE |
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