CRB3 binds directly to Par6 and regulates the morphogenesis of the tight junctions in mammalian epithelial cells
| Autor: | Emmanuelle Médina, André Le Bivic, Didier Michel, Jean-Pierre Arsanto, Céline Lemmers, Lydie Lane-Guermonprez, Marie-Hélène Delgrossi |
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| Přispěvatelé: | Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), CONTENSIN, Magali |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
animal structures
Morphogenesis Receptors Nerve Growth Factor [SDV.BC]Life Sciences [q-bio]/Cellular Biology Receptor Nerve Growth Factor Tight Junctions 03 medical and health sciences Mice 0302 clinical medicine Dogs MBoC 20th Anniversary Favorites Laminin Two-Hybrid System Techniques Chlorocebus aethiops Animals Humans Intestinal Mucosa Microscopy Immunoelectron Molecular Biology [SDV.BC] Life Sciences [q-bio]/Cellular Biology Cells Cultured 030304 developmental biology Epithelial polarity 0303 health sciences CRB1 Membrane Glycoproteins biology Tight junction Cell Membrane Cell Polarity Epithelial Cells Cell Biology Articles Apical membrane Transmembrane protein Cell biology Intestines Cytoplasm COS Cells biology.protein 030217 neurology & neurosurgery Protein Binding |
| Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, 2004, 15, pp.1324-1333 Molecular Biology of the Cell, American Society for Cell Biology, 2004, 15, pp.1324-1333 |
| ISSN: | 1939-4586 |
| Popis: | Crumbs is an apical transmembrane protein crucial for epithelial morphogenesis in Drosophila melanogaster embryos. A protein with all the characteristics for a Crumbs homologue has been identified from patients suffering from retinitis pigmentosa group 12, but this protein (CRB1) is only expressed in retina and some parts of the brain, both in human and mouse. Here, we describe CRB3, another Crumbs homologue that is preferentially expressed in epithelial tissues and skeletal muscles in human. CRB3 shares the conserved cytoplasmic domain with other Crumbs but exhibits a very short extracellular domain without the EGF- and laminin A-like G repeats present in the other Crumbs. CRB3 is localized to the apical and subapical area of epithelial cells from the mouse and human intestine, suggesting that it could play a role in epithelial morphogenesis. Indeed, expression of CRB3 or of a chimera containing the extracellular domain of the neurotrophin receptor p75NTR and the transmembrane and cytoplasmic domains of CRB3 led to a slower development of functional tight junctions in Madin-Darby canine kidney cells. This phenotype relied on the presence of CRB3 four last amino acids (ERLI) that are involved in a direct interaction with Par6, a regulator of epithelial polarity and tight junction formation. Thus, CRB3, through its cytoplasmic domain and its interactors, plays a role in apical membrane morphogenesis and tight junction regulation. |
| Databáze: | OpenAIRE |
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