Nucleotide sequence of the F plasmid gene, traC, and identification of its product
| Autor: | Kimberly A. Schandel, Robert E. Webster, Karin Ippen-Ihler, Raymond A. Vonder Haar, Sumit Maneewannakul |
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| Rok vydání: | 1990 |
| Předmět: |
Genetics
Transition (genetics) Base Sequence Operon Molecular Sequence Data Nucleic acid sequence TRAC General Medicine Molecular cloning Biology Molecular biology F Factor Plasmid Bacterial Proteins Protein Biosynthesis Coding region Amino Acid Sequence Fimbriae Proteins Gene computer computer.programming_language Plasmids |
| Zdroj: | Gene. 96(1) |
| ISSN: | 0378-1119 |
| Popis: | The traC gene of the F plasmid tra operon is required for the assembly of mature F-pilin subunits into extended F pili. The nucleotide sequence of traC was determined with a determined with a deduced coding region of 875 amino acids (aa) and 99066 Da. The traC1044 mutant allele, which allows filamentous phage infection in the absence of piliation, contains a C-to-T transition leading to an Arg----Cys substitution. Confirmation of the translational start came from the direct N-terminal aa sequencing of a TraC-alkaline phosphatase fusion protein. |
| Databáze: | OpenAIRE |
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