Posterior Oropharyngeal Saliva for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
| Autor: | Tak Chiu Wu, David Christopher Lung, Johnny Wai Man Chan, Cindy W. S. Tse, Sally Cheuk-Ying Wong, Tsz Shan Kwong, Wah Cheuk, Kwok Chi Cheung, Hon Kei Siu, Felix Yat Sun Yau, Herman Tse, Kin Chiu Poon, Man Yee Chu, Ingrid Y.Y. Cheung |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
mass screening Microbiology (medical) medicine.medical_specialty Saliva 2019-20 coronavirus outbreak Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Oropharynx medicine.disease_cause Gastroenterology Mean difference Specimen Handling 03 medical and health sciences 0302 clinical medicine Internal medicine Correspondence Major Article Medicine Humans 030212 general & internal medicine Respiratory system Pandemics Mass screening Coronavirus Retrospective Studies business.industry Clinical Laboratory Techniques SARS-CoV-2 pandemic COVID-19 Retrospective cohort study Virology 030104 developmental biology Infectious Diseases AcademicSubjects/MED00290 business severe acute respiratory syndrome coronavirus 2 |
| Zdroj: | Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America Clinical Infectious Diseases |
| ISSN: | 1537-6591 |
| Popis: | Background The coronavirus disease 2019 (COVID-19) pandemic has put tremendous pressure on the healthcare system worldwide. Diagnostic testing remained one of the limiting factors for early identification and isolation of infected patients. This study aimed to evaluate posterior oropharyngeal saliva (POPS) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection among patients with confirmed or suspected COVID-19. Methods The laboratory information system was searched retrospectively for all respiratory specimens and POPS requested for SARS-CoV-2 RNA detection between 1 February 2020 and 15 April 2020. The agreement and diagnostic performance of POPS against NPsp were evaluated. Results A total of 13772 specimens were identified during the study period, including 2130 POPS and 8438 nasopharyngeal specimens (NPsp). Two hundred and twenty-nine same-day POPS-NPsp paired were identified with POPS and NPsp positivity of 61.5% (95% confidence interval [CI] 55.1–67.6%) and 53.3% (95% CI 46.8–59.6%). The overall, negative and positive percent agreement were 76.0% (95% CI 70.2–80.9%), 65.4% (95% CI 55.5–74.2%), 85.2% (95% CI 77.4–90.8%). Better positive percent agreement was observed in POPS-NPsp obtained within 7 days (96.6%, 95% CI 87.3–99.4%) compared with after 7 days of symptom onset (75.0%, 95% CI 61.4–85.2%). Among the 104 positive pairs, the mean difference in Cp value was 0.26 (range: 12.63 to −14.74), with an overall higher Cp value in NPsp (Pearson coefficient 0.579). No significant temporal variation was noted between the 2 specimen types. Conclusions POPS is an acceptable alternative specimen to nasopharyngeal specimen for the detection of SARS-CoV-2. |
| Databáze: | OpenAIRE |
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