Inhibition of hsa_circ_0002570 suppresses high-glucose-induced angiogenesis and inflammation in retinal microvascular endothelial cells through miR-1243/angiomotin axis
| Autor: | Xuchen Ding, Miao Tian, Guo-Dan Liu, Shi-Feng Zhou, Xinge Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Angiogenesis Inflammation Biochemistry Cell Line 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Western blot microRNA medicine Humans Tube formation Messenger RNA Original Paper Diabetic Retinopathy medicine.diagnostic_test Neovascularization Pathologic Chemistry Microfilament Proteins Endothelial Cells Cell Biology RNA Circular Angiomotin MicroRNAs 030104 developmental biology Angiomotins 030220 oncology & carcinogenesis Cancer research Intercellular Signaling Peptides and Proteins medicine.symptom |
| Zdroj: | Cell Stress Chaperones |
| ISSN: | 1466-1268 |
| Popis: | Diabetic retinopathy (DR) is the most severe microvascular complication of diabetes and a major cause of visual impairment and blindness. However, the treatment for DR is still limited. Our study aimed to explore the role of circular RNA_0002570 in DR. First, we predicted the potential microRNA and mRNA that could bind to circ_0002570 and identified the miR-1243 and angiomotin gene; then, we used RT-PCR and Western blot to measure their expression. Next, we evaluated the abilities of proliferation, migration, and angiogenesis in vitro in human retinal microvascular endothelial cells (hRMECs) by CCK-8, transwell assay, and tube formation assay, respectively. To analyze the relationship among miR-1243, circ_0002570, and angiomotin, RNA pull-down and luciferase assay were performed. Our results showed that, in DR patients and high-glucose–induced hRMECs, miR-1243, circ_0002570, and angiomotin were all abnormally expressed. MiR-1243 could directly and competitively bind to both circ_0002570 and angiomotin mRNA to inhibit their expression. Moreover, circ_0002570 suppressed the abilities of proliferation, migration, and angiogenesis in hRMECs induced by high glucose, which was dependent on miR-1243-angiomotin axis. Furthermore, circ_0002570 could upregulate angiomotin by targeting miR-1243 to mediate the dysfunction of hRMECs induced by high glucose. In conclusion, circ_0002570 might serve as a potential target for diagnosis and treatment for DR. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink