Physiological Reports
| Autor: | Hao Shi, David E. Gerrard, Laila T. Kirkpatrick, Con-Ning Yen, Ashley E. Geiger, Morgan R. Daughtry |
|---|---|
| Přispěvatelé: | Animal and Poultry Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
obesity
medicine.medical_specialty Satellite Cells Skeletal Muscle Physiology Muscle Fibers Skeletal Cell Apoptosis Inflammation 030204 cardiovascular system & hematology Diet High-Fat lcsh:Physiology Mice 03 medical and health sciences 0302 clinical medicine Physiology (medical) Internal medicine satellite cell medicine Animals Regeneration Obesity Cells Cultured Original Research muscle regeneration lcsh:QP1-981 biology Metabolic disorder Skeletal muscle medicine.disease biology.organism_classification In vitro Mice Inbred C57BL Muscle regeneration Endocrinology medicine.anatomical_structure Satellite (biology) medicine.symptom 030217 neurology & neurosurgery |
| Zdroj: | Physiological Reports Physiological Reports, Vol 8, Iss 15, Pp n/a-n/a (2020) |
| Popis: | Obesity is a complex metabolic disorder that often leads to a decrease in insulin sensitivity, chronic inflammation, and overall decline in human health and well‐being. In mouse skeletal muscle, obesity has been shown to impair muscle regeneration after injury; however, the mechanism underlying these changes has yet to be determined. To test whether there is a negative impact of obesity on satellite cell (SC) decisions and behaviors, we fed C57BL/6 mice normal chow (NC, control) or a high‐fat diet (HFD) for 10 weeks and performed SC proliferation and differentiation assays in vitro. SCs from HFD mice formed colonies with smaller size (p .05) of the regenerating fibers in HFD and NC muscles, suggesting that other factors may mitigate the negative impact of obesity on SCs properties. In non‐injured muscle, muscle satellite (stem) cells from mice fed a high fat diet (HFD) exhibited reduced capacity in proliferation, differentiation, and quiescence maintenance, with a concomitant increase in apoptosis. When muscle was injured, HFD muscle recovered to an extent comparable to the lean muscle, suggesting in vivo mechanisms may exist, perhaps at the whole body level, that can mitigate or overcome damage to muscle satellite cells. |
| Databáze: | OpenAIRE |
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