Purification of immunoreactive radiolabeled monoclonal antibodies with anti-idiotypic monoclonal antibodies
| Autor: | Serenella M. Pupa, Soldano Ferrone, Massimo Temponi |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Idiotype
Gel electrophoresis biology Chemistry medicine.drug_class Immunology Antibodies Monoclonal Idiotopes Monoclonal antibody Molecular biology Chromatography Affinity Antibodies Anti-Idiotypic Iodine Radioisotopes Affinity chromatography Cell culture biology.protein medicine Immunologic Techniques Tumor Cells Cultured Immunology and Allergy Hybridoma technology Humans Electrophoresis Polyacrylamide Gel Antibody |
| Zdroj: | Journal of immunological methods. 126(2) |
| ISSN: | 0022-1759 |
| Popis: | A method is described to purify immunoreactive monoclonal antibodies from radiolabeled monoclonal antibody preparations. The method is based on incubation of radiolabeled monoclonal antibodies with insolubilized anti-idiotypic monoclonal antibodies to idiotopes within the antigen-combining site of monoclonal antibodies to be purified and elution of bound monoclonal antibodies with a low pH buffer. The immunoreactive fraction of the purified monoclonal antibodies was at least 82%; the yield was at least 73%. The purification procedure did not cause any detectable change in the affinity constant of the eluted monoclonal antibodies. The method is simple and rapid; the requirement for anti-idiotypic monoclonal antibodies to idiotopes within the antigen-combining site of the antibodies to be purified is not likely to represent a major limitation in the broad application of the present method, since the hybridoma technology has greatly facilitated the development of anti-idiotypic monoclonal antibodies. |
| Databáze: | OpenAIRE |
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