Diagnostic imaging in near‐infrared photoimmunotherapy using a commercially available camera for indocyanine green
Autor: | Ryuhei Okada, Hisataka Kobayashi, Aki Furusawa, Peter L. Choyke, Hiroaki Wakiyama, Takuya Kato, Fuyuki Inagaki, Daiki Fujimura |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Cancer Research Fluorescence-lifetime imaging microscopy Immunoconjugates Indoles photoimmunotherapy Materials science Nir light ICG camera Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor Neoplasms Report Medical imaging Animals Humans Organosilicon Compounds IR700 Photosensitizing Agents Optical Imaging Near-infrared spectroscopy Photoimmunotherapy General Medicine Phototherapy Trastuzumab Combined Modality Therapy Xenograft Model Antitumor Assays Fluorescence near‐infrared Tumor detection 030104 developmental biology Oncology chemistry 030220 oncology & carcinogenesis Female Immunotherapy Indocyanine green Reports Biomedical engineering |
Zdroj: | Cancer Science |
ISSN: | 1349-7006 1347-9032 |
Popis: | Near‐infrared photoimmunotherapy (NIR‐PIT) is a new type of cancer treatment, which was recently approved in Japan for patients with inoperable head and neck cancer. NIR‐PIT utilizes antibody‐IRDye700DX (IR700) conjugates and NIR light at a wavelength of 690 nm. NIR light exposure leads to physicochemical changes in the antibody‐IR700 conjugate cell receptor complex, inducing rapid necrotic cell death. Just as fluorescence guided surgery is useful for surgeons to resect tumors completely, real‐time information of tumor locations would help clinicians irradiate NIR light more precisely. IR700 is a fluorescence dye that emits at 702 nm; however, there is no clinically available device optimized for detecting this fluorescence. On the other hand, many indocyanine green (ICG) fluorescence imaging devices have been approved for clinical use. Therefore, we investigated whether LIGHTVISION, one of the clinically available ICG cameras, could be employed for tumor detection. We hypothesized that irradiation with even low‐power 690‐nm laser light, attenuated by 99% with a neutral‐density filter, could be detected with LIGHTVISION without fluorescence decay or therapeutic effect because of the long emission tail of IR700 beyond 800 nm (within the detection range of LIGHTVISION). We demonstrated that the LIGHTVISION camera, originally designed for ICG detection, can detect the tail of IR700 fluorescence in real time, thus enabling the visualization of target tumors. A diagnostic imaging method is developed for accurately localizing tumor and IR700 accumulation based on IR700 fluorescence, using weakened therapeutic laser light at a nontherapeutic dose and a commercially available clinical fluorescence camera for indocyanine green (LIGHTVISION). |
Databáze: | OpenAIRE |
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