An innovative genosensor for the monitoring of Leishmania spp sequence using binding of pDNA to cDNA based on Cit-AgNPs
| Autor: | Nasrin Razmi, Parina Mehri, Paria Pashazadeh-Panahi, Mohammad Hasanzadeh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Bioanalysis Nanostructure Spectrofluorimetric Article Analytical Chemistry Microbiology Analytical chemistry Nanotechnology Affinity binding Leishmaniasis Spectrophotometer Biosensing 03 medical and health sciences 0302 clinical medicine Complementary DNA parasitic diseases Analytisk kemi medicine lcsh:Social sciences (General) lcsh:Science (General) Pathogen Sequence (medicine) Multidisciplinary biology Chemistry Hybridization probe medicine.disease Leishmania biology.organism_classification 030104 developmental biology lcsh:H1-99 Biosensor 030217 neurology & neurosurgery lcsh:Q1-390 |
| Zdroj: | Heliyon, Vol 6, Iss 8, Pp e04638-(2020) Heliyon |
| ISSN: | 2405-8440 |
| Popis: | Leishmaniasis considered as the most crucial epidemic-prone diseases according to the World Health Organization. Early diagnoses and therapy of Leishmania infection is a great challenge since, it has no symptom and is resistance to drugs. Therefore, there is an urgent need for sensitive and precise detection of this pathogen. In this study, a new method was developed for optical biosensing of Leishmania spp sequence based on hybridization of Citrate capped Ag nanoparticles bonded to specific single stranded DNA probe of Leishmania spp. Aggregation of the Citrate capped Ag nanoparticles in the existence or lack of a cDNA sequence of Leishmania, cause eye catching and considerable significant alter in the UV–vis. The obtained low limit of quantification (LLOQ) of was achieved as 1ZM. Based on experimental results in optimum conditions, quick bioanalysis of Leishmania spp sequence was performed (2 min). So, this probe can be used for the clinical diagnosis of this pathogen and infection disease. Analytical chemistry; Nanotechnology, Nanostructure; Affinity binding; Leishmaniasis; Spectrophotometer; Spectrofluorimetric; Biosensing |
| Databáze: | OpenAIRE |
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