Comet assay and micronucleus tests on Oreochromis niloticus (Perciforme: Cichlidae) exposed to raw sugarcane vinasse and to phisicochemical treated vinasse by pH adjustment with lime (CaO)
| Autor: | Cintya Ap. Christofoletti, Carmem Silvia Fontanetti, Thays de Andrade Guedes, Jorge E. Correia, Yadira Ansoar-Rodríguez |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Environmental Engineering Serial dilution Health Toxicology and Mutagenesis Vinasse Industrial Waste 010501 environmental sciences engineering.material medicine.disease_cause 01 natural sciences 03 medical and health sciences Soil medicine Environmental Chemistry Bioassay Animals Food science 0105 earth and related environmental sciences Lime Micronucleus Tests business.industry Chemistry Public Health Environmental and Occupational Health Oxides General Medicine General Chemistry Cichlids Calcium Compounds Hydrogen-Ion Concentration Pollution Biotechnology Saccharum Comet assay 030104 developmental biology Micronucleus test engineering Comet Assay Micronucleus business Genotoxicity Brazil DNA Damage |
| Zdroj: | Chemosphere. 173 |
| ISSN: | 1879-1298 |
| Popis: | In Brazil vinasse, a main sugarcane distillery residue, stands out because every liter of alcohol generates 10–15 L of vinasse as waste. An alternative for the disposal of this waste is the fertirrigation of the sugarcane culture itself. However, the high amount released can saturate the soil and through leaching/percolation contaminate water resources. The aim of this study is verifying the toxic potential of vinasse in tilapias and effectiveness of the physicalchemical treatment of this waste with pH adjustment with lime (CaO). The comet assay and the micronucleus test were applied on animals exposed to dilutions of raw vinasse and vinasse adjusted to neutral pH. Bioassays with raw vinasse dilutions indicated a toxic and genotoxic potential; fish exposed to the highest concentration died less than 48 h after the exposure; the incidence of micronucleus was significantly higher when compared to negative control for all dilutions. For the comet assay, the scores of damage were statistically higher for all dilutions, with the exception of the 1% dillution. However, in the bioassay with the chemically treated vinasse (neutral pH), most fish in the 10% dilution survived and there was no significant difference when compared to the control. Damage scores in the comet assay were similar to the results of the untreated vinasse. The chemical treatment of vinasse with lime to neutralize the pH proved to be an effective alternative for the toxicity reduction of this residue, since it reduced the mortality of fish at higher concentrations and the incidence of damage to DNA. |
| Databáze: | OpenAIRE |
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