Adipocyte Proteins and Storage of Endogenous Fatty Acids in Visceral and Subcutaneous Adipose Tissue in Severe Obesity
| Autor: | Michael L. Kendrick, Todd A. Kellogg, James M. Swain, Nicola Gathaiya, Kazanna C. Hames, Michael D. Jensen, Jessica M. Triay, Kelli A Lytle, Nikki C. Bush |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty Very low-density lipoprotein Adolescent Endocrinology Diabetes and Metabolism Subcutaneous Fat Bariatric Surgery Medicine (miscellaneous) Adipose tissue 030209 endocrinology & metabolism Fatty Acids Nonesterified Intra-Abdominal Fat Lipoproteins VLDL Article Young Adult 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Internal medicine Adipocyte Adipocytes medicine Humans Storage protein 030212 general & internal medicine Triglycerides chemistry.chemical_classification Lipoprotein lipase Nutrition and Dietetics Fatty Acids nutritional and metabolic diseases Fatty acid Middle Aged Obesity Morbid Adipose Tissue chemistry Acyltransferase Female lipids (amino acids peptides and proteins) Lipoprotein |
| Zdroj: | Obesity (Silver Spring) |
| ISSN: | 1930-739X 1930-7381 |
| Popis: | OBJECTIVE This study tested whether substrate concentrations or fatty acid storage proteins predict storage of endogenous lipids in visceral adipose tissue (VAT) and upper body subcutaneous adipose tissue (UBSQ) fat. METHODS The day prior to surgery, 25 patients undergoing bariatric procedures received an infusion of autologous [1-14 C]triolein-labeled very low-density lipoprotein (VLDL) particles, and during surgery, they received a continuous [U-13 C]palmitate infusion/bolus [9,10-3 H]palmitate tracer. VAT and UBSQ fat were collected to measure VLDL-triglyceride (TG) storage, direct free fatty acid (FFA) storage rates, CD36 content, lipoprotein lipase (LPL), acyl-CoA synthetase, diacylglycerol acetyl-transferase, and glycerol-3-phosphate acyltransferase activities. RESULTS Storage of VLDL-TG and FFA-palmitate in UBSQ and VAT was not different. Plasma palmitate concentrations correlated with palmitate storage rates in UBSQ and VAT (r = 0.46, P = 0.02 and r = 0.46, P = 0.02, respectively). In VAT, VLDL-TG storage was correlated with VLDL concentrations (r = 0.53, P < 0.009) and LPL (r = 0.42, P < 0.05). In UBSQ, VLDL-TG storage was correlated with LPL (r = 0.42, P < 0.05). CD36, acyl-CoA synthetase, glycerol-3-phosphate acyltransferase, and diacylglycerol acetyl-transferase were not correlated with VLDL-TG or palmitate storage. CONCLUSIONS Adipose storage of VLDL-TG is predicted by VLDL-TG concentrations and LPL; FFA concentrations predict direct adipose tissue FFA storage rates. |
| Databáze: | OpenAIRE |
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