The LIM-only protein, LMO4, and the LIM domain-binding protein, LDB1, expression in squamous cell carcinomas of the oral cavity
| Autor: | Kazushi Imai, K Sanada, H Mizunuma, J Miyazawa |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Cancer Research
Somatic cell LDB1 Cellular differentiation Clone (cell biology) carcinoma Biology Polymerase Chain Reaction Tumor Cells Cultured Carcinoma medicine Humans Neoplasm Epigenetics Neoplasm Metastasis DNA Primers Cell Nucleus Oncogene Proteins Mouth neoplasm LIM Molecular and Cellular Pathology Cell Differentiation LIM Domain Proteins medicine.disease DNA-Binding Proteins Cell Transformation Neoplastic Oncology Epidermoid carcinoma Carcinoma Squamous Cell Disease Progression Cancer research Mouth Neoplasms LMO4 Transcription Factors |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/sj.bjc.6600952 |
| Popis: | Carcinoma cells can lose their epithelial cell characteristics and dedifferentiate into a fibroblast-like cell during progression of a neoplasm. Aberrant expression of oligomeric transcriptional complexes contributes to progression of carcinomas. Although individual transcription factors initiating progression remain unknown, LIM-only protein (LMO) and LIM-domain binding protein (LDB) negatively regulate breast carcinoma cell differentiation. In this study, we investigated the expression of LMO4 and LDB in squamous cell carcinomas of the oral cavity. LMO4 mRNA was amplified in four of six carcinoma tissues and eight of 12 carcinoma cell lines, and LDB1 in three carcinoma tissues and 11 cell lines examined. Immunoprecipitation studies revealed that LMO4 and LDB1 interact with each other in the nuclear milieu of the carcinoma cells indicating the presence of an LMO4-LDB1-mediated transcription complex. Both LMO4 and LDB1 proteins were preferentially localised in the nuclei of carcinoma cells at the invasive front and the immunoreactivity was increased in less-differentiated carcinoma tissues (P0.01). Carcinoma cells metastasised to the cervical lymph nodes with increased immunoreactivity compared to the primary site of neoplasm (P0.05). These data suggest that the LMO4-LDB1 complexes may be involved in carcinoma progression possibly through dedifferentiation of squamous carcinoma cells of the oral cavity. |
| Databáze: | OpenAIRE |
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