Holobionts and ecological speciation: the intestinal microbiota of lake whitefish species pairs
| Autor: | Louis Bernatchez, Nicolas Derome, Maelle Sevellec |
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| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Microbiology (medical) Coregonus clupeaformis Zoology digestive system Microbiology lcsh:Microbial ecology Ecological speciation 03 medical and health sciences Whitefish intestinal microbiota-speciation Microbial ecology RNA Ribosomal 16S Animals Bacteria Base Sequence biology Research Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA Gastrointestinal Microbiome Intestines Holobiont Lakes stomatognathic diseases 030104 developmental biology Sympatric speciation Benthic zone lcsh:QR100-130 Salmonidae Limnetic zone |
| Zdroj: | Microbiome, Vol 6, Iss 1, Pp 1-15 (2018) Microbiome |
| ISSN: | 2049-2618 |
| DOI: | 10.1186/s40168-018-0427-2 |
| Popis: | Background It is well established that symbionts have considerable impact on their host, yet the investigation of the possible role of the holobiont in the host’s speciation process is still in its infancy. In this study, we compared the intestinal microbiota among five sympatric pairs of dwarf (limnetic) and normal (benthic) lake whitefish Coregonus clupeaformis representing a continuum in the early stage of ecological speciation. We sequenced the 16s rRNA gene V3-V4 regions of the intestinal microbiota present in a total of 108 wild sympatric dwarf and normal whitefish as well as the water bacterial community from five lakes to (i) test for differences between the whitefish intestinal microbiota and the water bacterial community and (ii) test for parallelism in the intestinal microbiota of dwarf and normal whitefish. Results The water bacterial community was distinct from the intestinal microbiota, indicating that intestinal microbiota did not reflect the environment, but rather the intrinsic properties of the host microbiota. Our results revealed a strong influence of the host (dwarf or normal) on the intestinal microbiota with pronounced conservation of the core intestinal microbiota (mean ~ 44% of shared genera). However, no clear evidence for parallelism was observed, whereby non-parallel differences between dwarf and normal whitefish were observed in three of the lakes while similar taxonomic composition was observed for the two other species pairs. Conclusions This absence of parallelism across dwarf vs. normal whitefish microbiota highlighted the complexity of the holobiont and suggests that the direction of selection could be different between the host and its microbiota. Electronic supplementary material The online version of this article (10.1186/s40168-018-0427-2) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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