Design and evaluation of an in-house HIV-1 (group M and O), SIVmnd and SIVcpz antigen capture assay
| Autor: | A Bouckaert, Els Beirnaert, Peter M. Ndumbe, M. Peeters, K Vereecken, Leo Heyndrickx, Wouter Janssens, G van der Groen, Sandra Coppens, Ping Zhong, B. Willems, David A. Davis |
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| Rok vydání: | 1998 |
| Předmět: |
HIV Antigens
Blotting Western HIV Core Protein p24 Human immunodeficiency virus (HIV) Enzyme-Linked Immunosorbent Assay medicine.disease_cause Sensitivity and Specificity Virus Antigen Virology medicine Humans Antigens Viral biology Immune Sera Antibodies Monoclonal virus diseases Elisa assay Simian immunodeficiency virus Antigen capture assay biology.organism_classification Immunoglobulin G HIV-2 Lentivirus HIV-1 Simian Immunodeficiency Virus |
| Zdroj: | Journal of Virological Methods. 73:65-70 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/s0166-0934(98)00044-5 |
| Popis: | An enzyme-linked immuno-sorbent assay (ELISA) for the detection of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIVcpz/SIVmnd) antigens was designed using immunoreagents from naturally infected individuals, and compared to the commercially available Vironostika HIV-1 Antigen Microelisa System (Organon Teknika). The in-house assay proved to be specific for HIV-1 isolates belonging to group M (A-H) and group O and for SIVcpz and SIVmnd isolates, but was less sensitive than the Vironostika HIV-1 Antigen Microelisa System, except for SIVmnd. For the strains belonging to HIV-2, SIVmac and SIVagm, the in-house assay could not detect antigen to an appreciable degree. This study shows that a considerably less expensive but sufficiently accurate HIV-1 antigen capture assay can be developed to monitor HIV-1 (group M and O), SIVcpz and SIVmnd antigen in the supernatants of virus cultures. |
| Databáze: | OpenAIRE |
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