Effect of high K+ exposure on phosphoinositide metabolism in frog skeletal muscle
| Autor: | Zheng Dong, Pei Hong Zhu, Ke Ying Chen |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
medicine.medical_specialty
Contraction (grammar) Ranidae Potassium Biophysics chemistry.chemical_element In Vitro Techniques Biology Phosphatidylinositols Tritium Biochemistry chemistry.chemical_compound Salientia Internal medicine medicine Pi Animals Inositol Phosphatidylinositol Molecular Biology Muscles Cell Biology Metabolism biology.organism_classification Kinetics Endocrinology chemistry |
| Zdroj: | Biochemical and Biophysical Research Communications. 182:767-772 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(92)91798-u |
| Popis: | Using [3H]myo-inositol labeled frog skeletal muscles, we have studied the effect of high K+ exposure on phosphoinositide metabolism. After 12 hours labeling, 80mM K+ exposure induced a time-dependent change. The labeling associated with phosphatidylinositol (PI) and phosphatidylinositol 4-phosphate (PIP) gradually increased and decreased, respectively. The labeled phosphatidylinositol 4,5-bisphosphate (PIP2) first decreased, and then recovered. An accumulation of the labeling in inositol phosphates was shown. In shortening the labeling to 30 min, 15 min high K+ exposure was found to only increase the labeling in all fractions. Taken together, these results show that high K+ exposure can activate the turnover of phosphoinositides, which is consistent with the hypothesis that the metabolism of phosphoinositides may regulate excitation- contraction (e-c) coupling. |
| Databáze: | OpenAIRE |
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