Lack of Islet Amyloid Polypeptide Regulation of Insulin Biosynthesis or Secretion in Normal Rat Islets
| Autor: | Gerold M. Grodsky, Raymond J. Carroll, Shinya Nagamatsu, Donald F. Steiner |
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| Rok vydání: | 1990 |
| Předmět: |
Male
Amyloid endocrine system medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism medicine.medical_treatment In Vitro Techniques Biology Islets of Langerhans chemistry.chemical_compound Biosynthesis Diabetes mellitus Internal medicine Insulin Secretion Internal Medicine medicine Animals Insulin Secretion Proinsulin geography geography.geographical_feature_category Rats Inbred Strains medicine.disease Islet Islet Amyloid Polypeptide Rats Insulin oscillation Kinetics Glucose Endocrinology chemistry Protein Biosynthesis |
| Zdroj: | Diabetes. 39:871-877 |
| ISSN: | 1939-327X 0012-1797 |
| DOI: | 10.2337/diab.39.7.871 |
| Popis: | We examined the effects of rat islet amyloid polypeptide (IAPP) on insulin biosynthesis and secretion by isolated rat islets of Langerhans. Culture of islets for 24 h in the presence of 10−6 M IAPP and 5.5 mM glucose had no effect on insulin mRNA levels. Similarly, the rates of proinsulin biosynthesis were not altered in islets incubated in 10−4–10−9 M IAPP and 5.5 mM glucose, nor was the rate of conversion of proinsulin to insulin changed at 10−6 M IAPP. Addition of 10−5 M IAPP to islets incubated in 11 mM glucose decreased the fractional insulin secretion rate; however, the secretion of newly synthesized proinsulin and insulin was not affected. These data indicate that it is unlikely that IAPP is a physiologically relevant modulator of insulin biosynthesis or secretion. |
| Databáze: | OpenAIRE |
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