In vivo imaging of sentinel nodes using fluorescent silica nanoparticles in living mice
| Autor: | Dong Soo Lee, Yong Hyun Jeon, Yun Sang Lee, Jaetae Lee, Bo Quan, Jing Yu Piao, Young-Hwa Kim, Myung Chul Lee, Keon Wook Kang, Kihwan Choi, Jae Min Jeong, June-Key Chung, Doo Soo Chung |
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| Rok vydání: | 2008 |
| Předmět: |
Cancer Research
Biodistribution Brachial lymph nodes Sentinel lymph node Gallium Heterocyclic Compounds 1-Ring Mice Heterocyclic Compounds medicine Animals Frozen Sections Radiology Nuclear Medicine and imaging Tissue Distribution Lymph node Mice Inbred BALB C Chemistry Rhodamines Extremities Sentinel node Silicon Dioxide Fluorescence Molecular Imaging Lymphatic system medicine.anatomical_structure Spectrometry Fluorescence Oncology Microscopy Fluorescence Nanoparticles Female Lymph Nodes Preclinical imaging Biomedical engineering |
| Zdroj: | Molecular imaging and biology. 12(2) |
| ISSN: | 1860-2002 |
| Popis: | We examine the feasibility of fluorescent imaging system for sentinel lymph node detection by using functionalized silica nanoparticles.We developed a functionalized RITC-SiO(2) nanoparticles containing fluorescent dye, C(28)H(31)N(2)O(3)Cl (rhodamine B isothiocyanate) inside, and subsequently synthesized (68)Ga-NOTA-RITC-SiO(2) nanoparticles.At 5 min after RITC-doped silica nanoparticles injection, fluorescent signals were shown in both right axillary lymph node (ALN) and injection site of living mice. Fluorescent signals were also observed at these locations in a biodistribution study. In addition, fluorescence was detected in frozen ALN sections microscopically. The percentages of doses injected per gram of tissue of axillary and brachial lymph nodes near footpad treated with (68)Ga-NOTA-RITC-SiO(2) nanoparticles were 308.3 +/- 3.4 and 41.5 +/- 6.1, respectively. Little (68)Ga radioactivity was found in other organs.Our data provide strong evidence that functionalized silica nanoparticles has a promising potential as organic lymphatic tracer in biomedical imaging such as pre- and intraoperative surgical guidance. |
| Databáze: | OpenAIRE |
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