Evaluation of four rapid tests for diagnosis and differentiation of HIV-1 and HIV-2 infections in Guinea-Conakry, West Africa
| Autor: | Katrien Fransen, Katie Tayler-Smith, Rony Zachariah, Greet Beelaert, Nanfack Duclos, Diallo Moctar, Pascale Chaillet |
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| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Africa West Efficacy Human immunodeficiency virus (HIV) HIV Infections Viral diseases HIV Antibodies medicine.disease_cause Sensitivity and Specificity Sensitivity Acquired immunodeficiency syndrome (AIDS) Internal medicine Medicine Humans Sida Evaluation Accuracy biology business.industry Rapid diagnostic tests Public Health Environmental and Occupational Health virus diseases General Medicine Gold standard (test) Guinea conakry biology.organism_classification medicine.disease Virology AIDS Africa Western Infectious Diseases Differentiation Lentivirus Tropical medicine HIV-2 Specificity HIV-1 Parasitology Guinea Female Viral disease Reagent Kits Diagnostic business Algorithms |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 104(9) |
| ISSN: | 1878-3503 |
| Popis: | With both HIV-1 and HV-2 prevalent in Guinea-Conakry, accurate diagnosis and differentiation is crucial for treatment purposes. Thus, four rapid HIV tests were evaluated for their HIV-1 and HIV-2 diagnostic and discriminative capacity for use in Guinea-Conakry. These included SD Bioline HIV 1/2 3.0 (Standard Diagnostics Inc.), Genie II HIV1/HIV2 (Bio-Rad), First Response HIV Card Test 1-2.0 (PMC Medical) and Immunoflow HIV1-HIV2 (Core Diagnostics). Results were compared with gold standard tests (INNO-LIA HIV-I/II Score) and NEW LAV BLOT II (Bio-Rad). Four hundred and forty three sequential stored HIV-positive serum samples, of known HIV-type, were evaluated. Genie II HIV1/HIV2, Immunoflow HIV1-HIV2 and SD Bioline HIV 1/2 3.0 had 100% sensitivity (95% CI, 98.9-100%) while for First Response HIV Card Test 1-2.0 this was 99.5% (95% CI, 98.2%-99.9%). In terms of discriminatory capacity, Genie II HIV1/HIV2 identified 382/ 384(99.5%) HIV-1 samples, 49/ 52(95%) HIV-2 and 7/7(100%) HIV-positive untypable samples. Immunoflow HIV1-HIV2 identified 99% HIV-1, 67% HIV-2 and all HIV-positive untypable samples. First Response HIV Card Test 1-2.0 identified 94% HIV-1, 64% HIV-2 and 57% HIV-positive untypable samples. SD-Bioline HIV 1/2 3.0 was the worst overall performer identifying 65% HIV-1, 69% HIV-2 and all HIV-positive untypable samples. The use of SD Bioline HIV 1/2 3.0 (the current standard in Guinea-Conakry) as a discriminatory HIV test is poor and may be best replaced by Immunoflow HIV1-HIV2. |
| Databáze: | OpenAIRE |
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