Compound Transfer by Acoustic Droplet Ejection Promotes Quality and Efficiency in Ultra-High-Throughput Screening Campaigns
| Autor: | Dhireshan Gadiagellan, Amy Gustafson, Daniel P. Gross, Timothy D. Dawes, Till Maurer, Peter Thana, Linda Orren, Rebecca Turincio, Richard A. Rodriguez, Marya Liimatta, Maureen Beresini, Steven W. Jones, Kevin R Clark |
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| Rok vydání: | 2016 |
| Předmět: |
Engineering
High-throughput screening media_common.quotation_subject Biomedical Technology Drug Evaluation Preclinical 01 natural sciences Small Molecule Libraries Disk formatting Transfer (computing) Quality (business) Instrumentation (computer programming) Process engineering Acoustic droplet ejection Simulation media_common business.industry 010401 analytical chemistry Acoustics High-Throughput Screening Assays 0104 chemical sciences Computer Science Applications Solutions 010404 medicinal & biomolecular chemistry Medical Laboratory Technology Data Interpretation Statistical Custom software business Software Primary screening |
| Zdroj: | SLAS Technology. 21:64-75 |
| ISSN: | 2472-6303 |
| Popis: | Acoustic droplet ejection (ADE) as a means of transferring library compounds has had a dramatic impact on the way in which high-throughput screening campaigns are conducted in many laboratories. Two Labcyte Echo ADE liquid handlers form the core of the compound transfer operation in our 1536-well based ultra-high-throughput screening (uHTS) system. Use of these instruments has promoted flexibility in compound formatting in addition to minimizing waste and eliminating compound carryover. We describe the use of ADE for the generation of assay-ready plates for primary screening as well as for follow-up dose-response evaluations. Custom software has enabled us to harness the information generated by the ADE instrumentation. Compound transfer via ADE also contributes to the screening process outside of the uHTS system. A second fully automated ADE-based system has been used to augment the capacity of the uHTS system as well as to permit efficient use of previously picked compound aliquots for secondary assay evaluations. Essential to the utility of ADE in the high-throughput screening process is the high quality of the resulting data. Examples of data generated at various stages of high-throughput screening campaigns are provided. Advantages and disadvantages of the use of ADE in high-throughput screening are discussed. |
| Databáze: | OpenAIRE |
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