A fate-mapping approach reveals the composite origin of the connecting tubule and alerts on 'single-cell'-specific KO model of the distal nephron
| Autor: | Anna Iervolino, Federica Petrillo, Günther Schütz, Juliette Hadchouel, Francesco Trepiccione, Giovambattista Capasso, Christelle Soukaseum, Dominique Eladari, Miriam Zacchia |
|---|---|
| Přispěvatelé: | Trepiccione, Francesco, Soukaseum, Christelle, Iervolino, Anna, Petrillo, Federica, Zacchia, Miriam, Schütz, Gunther, Eladari, Dominique, Capasso, Giovambattista, Hadchouel, Juliette |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell type Physiology Mice Transgenic Nephron Biology Models Biological Distal nephron Mice 03 medical and health sciences Fate mapping medicine Animals Connecting tubule Kidney Tubules Collecting Kidney Tubules Distal NCC Aquaporin 2 urogenital system Nephrons Anatomy AQP2 Sodium Chloride Symporters Epithelium 030104 developmental biology medicine.anatomical_structure Duct (anatomy) |
| Zdroj: | American Journal of Physiology-Renal Physiology. 311:F901-F906 |
| ISSN: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.00286.2016 |
| Popis: | The distal nephron is a heterogeneous part of the nephron composed by six different cell types, forming the epithelium of the distal convoluted (DCT), connecting, and collecting duct. To dissect the function of these cells, knockout models specific for their unique cell marker have been created. However, since this part of the nephron develops at the border between the ureteric bud and the metanephric mesenchyme, the specificity of the single cell markers has been recently questioned. Here, by mapping the fate of the aquaporin 2 (AQP2) and Na+-Cl−cotransporter (NCC)-positive cells using transgenic mouse lines expressing the yellow fluorescent protein fluorescent marker, we showed that the origin of the distal nephron is extremely composite. Indeed, AQP2-expressing precursor results give rise not only to the principal cells, but also to some of the A- and B-type intercalated cells and even to cells of the DCT. On the other hand, some principal cells and B-type intercalated cells can develop from NCC-expressing precursors. In conclusion, these results demonstrate that the origin of different cell types in the distal nephron is not as clearly defined as originally thought. Importantly, they highlight the fact that knocking out a gene encoding for a selective functional marker in the adult does not guarantee cell specificity during the overall kidney development. Tools allowing not only cell-specific but also time-controlled recombination will be useful in this sense. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|