Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells

Autor: Liisa M. Uotila, Celine Garcia, Susanna C. Fagerholm, Vicky L. Morrison, Andrew Filby, Jessica G. Borger, Rose Zamoyska, Fabio Simbari
Přispěvatelé: Institute of Biotechnology, Integrins in immunity
Rok vydání: 2017
Předmět:
0301 basic medicine
Immunological Synapses
IMMUNOLOGICAL SYNAPSE
T cell
Cellular polarity
Caveolin 1
INTEGRIN FUNCTION
Immunology
Receptors
Antigen
T-Cell
LEUKOCYTE ADHESION
CD8-Positive T-Lymphocytes
Biology
Lymphocyte Activation
Immunological synapse
Mice
03 medical and health sciences
Antigen Recognition and Responses
LYMPHOCYTE FUNCTION
Caveolin
medicine
Animals
Immunology and Allergy
Cytotoxic T cell
IL-2 receptor
IN-VIVO
ZAP70
Cell Membrane
T-cell receptor
ACTIN CYTOSKELETON
PHORBOL ESTER
Cell Polarity
Intercellular Adhesion Molecule-1
Lymphocyte Function-Associated Antigen-1
Sphingomyelins
Cell biology
LFA-1-DEFICIENT MICE
Cholesterol
030104 developmental biology
medicine.anatomical_structure
FUNCTION-ASSOCIATED MOLECULE-1
3121 General medicine
internal medicine and other clinical medicine
PLASMA-MEMBRANE
Signal Transduction
Zdroj: Borger, J, Morrison, V L, Filby, A, Garcia, C, Uotila, L M, Simbari, F, Fagerholm, S C & Zamoyska, R 2017, ' Caveolin-1 influences LFA-1 redistribution upon TCR stimulation in CD8 T cells ', Journal of Immunology, vol. 199, no. 3, pp. 874-884 . https://doi.org/10.4049/jimmunol.1700431
The Journal of Immunology Author Choice
ISSN: 1550-6606
0022-1767
DOI: 10.4049/jimmunol.1700431
Popis: TCR stimulation by peptide–MHC complexes on APCs requires precise reorganization of molecules into the area of cellular contact to form an immunological synapse from where T cell signaling is initiated. Caveolin (Cav)1, a widely expressed transmembrane protein, is involved in the regulation of membrane composition, cellular polarity and trafficking, and the organization of signal transduction pathways. The presence of Cav1 protein in T cells was identified only recently, and its function in this context is not well understood. We show that Cav1-knockout CD8 T cells have a reduction in membrane cholesterol and sphingomyelin, and upon TCR triggering they exhibit altered morphology and polarity, with reduced effector function compared with Cav1 wild-type CD8 T cells. In particular, redistribution of the β2 integrin LFA-1 to the immunological synapse is compromised in Cav1-knockout T cells, as is the ability of LFA-1 to form high-avidity interactions with ICAM-1. Our results identify a role for Cav1 in membrane organization and β2 integrin function in primary CD8 T cells.
Databáze: OpenAIRE
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