Direct physical contact between intercalated cells in the distal convoluted tubule and the afferent arteriole in mouse kidneys
| Autor: | Jesper Skovhus Thomsen, Ning-Yu Liu, Hao Ren, Liu Cao, Arne Andreasen, Xiao-Yue Zhai, Erik Ilsø Christensen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Afferent arterioles Anion Transport Proteins lcsh:Medicine Nephron Biology Mice medicine Animals Intercalated Cell Distal convoluted tubule Kidney Tubules Distal lcsh:Science Kidney Multidisciplinary urogenital system lcsh:R Anatomy Cell biology Mice Inbred C57BL Arterioles Crosstalk (biology) Intercellular Junctions medicine.anatomical_structure Tubule Sulfate Transporters Macula densa lcsh:Q Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 9, p e70898 (2013) Ren, H, Liu, N-Y, Andreasen, A, Thomsen, J S, Cao, L, Christensen, E I & Zhai, X-Y 2013, ' Direct Physical Contact between Intercalated Cells in the Distal Convoluted Tubule and the Afferent Arteriole in Mouse Kidneys ', PLOS ONE, vol. 8, no. 9, pp. e70898 . https://doi.org/10.1371/journal.pone.0070898 PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0070898 |
| Popis: | Recent physiological studies in the kidney proposed the existence of a secondary feedback mechanism termed ‘crosstalk’ localized after the macula densa. This newly discovered crosstalk contact between the nephron tubule and its own afferent arteriole may potentially revolutionize our understanding of renal vascular resistance and electrolyte regulation. However, the nature of such a crosstalk mechanism is still debated due to a lack of direct and comprehensive morphological evidence. Its exact location along the nephron, its prevalence among the different types of nephrons, and the type of cells involved are yet unknown. To address these issues, computer assisted 3-dimensional nephron tracing was applied in combination with direct immunohistochemistry on plastic sections and electron microscopy. ‘Random’ contacts in the cortex were identified by the tracing and excluded. We investigated a total of 168 nephrons from all cortical regions. The results demonstrated that the crosstalk contact existed, and that it was only present in certain nephrons (90% of the short-looped and 75% of the long-looped nephrons). The crosstalk contacts always occurred at a specific position – the last 10% of the distal convoluted tubule. Importantly, we demonstrated, for the first time, that the cells found in the tubule wall at the contact site were always type nonA-nonB intercalated cells. In conclusion, the present work confirmed the existence of a post macula densa physical crosstalk contact. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|