Immunolocalization of a Novel Collectin CL-K1 in Murine Tissues
| Autor: | Seongjae Jang, Mituko Fukuda, Itsuro Yoshida, Jun Fukuzawa, Kenichiro Mori, Nobutaka Wakamiya, Yasuhiko Suzuki, Toshikatsu Okumura, Katsuki Ohtani, Takayuki Yoshizaki, Yutaka Kohgo, Wataru Motomura, Naoki Nomura |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Histology Vascular smooth muscle Collectin Enzyme-Linked Immunosorbent Assay CHO Cells Polymerase Chain Reaction Antibodies Article Mice Cricetulus Cricetinae medicine Animals Humans RNA Messenger Gastrointestinal tract Messenger RNA Kidney Innate immune system biology Immunohistochemistry Collectins Cell biology Mice Inbred C57BL medicine.anatomical_structure Organ Specificity Immunology biology.protein Rabbits Anatomy Antibody |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 56:243-252 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1369/jhc.7a7312.2007 |
| Popis: | author We have recently identified a novel collectin, CL-K1, that may play a role in innate immunity as a member of the collectin family. In this study using mice, we investigated the tissue distribution of CL-K1 for better understanding of its pathophysiological relevance. Real-time PCR analyses demonstrated that CL-K1 mRNA was expressed in all tissues tested. Immunohistochemical analyses demonstrated that CL-K1 was expressed in proximal tubules of kidney, in mucosa of the gastrointestinal tract, and in bronchial glands of bronchioles similar to the localization of SP-A and SP-D in these pulmonary structures. Immunohistochemistry also showed that CL-K1 was highly expressed in hepatocytes around the central veins in liver, which suggests that murine CL-K1 might be mainly produced in theliver and secreted into the blood stream as is human CL-K1. CL-K1 was especially detected in vascular smooth muscle in several types of tissue. In addition, it was also expressed in intestinal Paneth cells, in mesangial cells of kidney, in pancreatic islet D cells, and in neurons of the brain. It is of interest that this profile of CL-K1 expression is unique among the collectins. Taken together, these histological findings may be useful for the understanding biological function of this novel collectin. |
| Databáze: | OpenAIRE |
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