Characterization of the salivary microbiome in healthy Thai children
Autor: | Sroisiri Thaweboon, Izumi Mashima, Frank A. Scannapieco, Tippanart Vichayanrat, Boonyanit Thaweboon, Citra Fragrantia Theodorea, Futoshi Nakazawa |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Saliva
lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 030231 tropical medicine Population Rothia dentocariosa microbiome medicine.disease_cause Veillonella parvula 03 medical and health sciences 0302 clinical medicine stomatognathic system children Prevotella medicine simplified oral hygiene index Microbiome education education.field_of_study saliva region biology Rothia aeria General Medicine biology.organism_classification thai stomatognathic diseases Metagenomics 030220 oncology & carcinogenesis Demography |
Zdroj: | Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 4, Pp 163-169 (2019) |
ISSN: | 2352-4146 |
Popis: | Objective: To investigate the composition of the salivary microbiome of 50 healthy Thai children. Methods: A total 76 provinces in Thailand are grouped into 5 geographical clusters based on unique economics, foods and lifestyles. Geographical locations and the results of an oral assessment were also considered. Genomic DNA was extracted from stimulated sdiva samples. Subsequently, amplicon libraries were prepared by 16S Metagenomic Sequencing Library Preparation. The amplicons were sequenced using an Illumina Miseq platform followed by bioinformatics and statistical analyses. Results: The correlation between oral hygiene status and caries history varied from r2=0.887 to r2=0.999 in the geographical groups, suggesting oral hygiene status a strong association between caries history. Twenty taxonomic groups were found in all subjects and constituted 93.6%-96.5% of the microbiome. Of these, genus Veillonella and Prevotella showed significant differences in their proportions between the geographical groups (P |
Databáze: | OpenAIRE |
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