Characterization of the salivary microbiome in healthy Thai children

Autor: Sroisiri Thaweboon, Izumi Mashima, Frank A. Scannapieco, Tippanart Vichayanrat, Boonyanit Thaweboon, Citra Fragrantia Theodorea, Futoshi Nakazawa
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 4, Pp 163-169 (2019)
ISSN: 2352-4146
Popis: Objective: To investigate the composition of the salivary microbiome of 50 healthy Thai children. Methods: A total 76 provinces in Thailand are grouped into 5 geographical clusters based on unique economics, foods and lifestyles. Geographical locations and the results of an oral assessment were also considered. Genomic DNA was extracted from stimulated sdiva samples. Subsequently, amplicon libraries were prepared by 16S Metagenomic Sequencing Library Preparation. The amplicons were sequenced using an Illumina Miseq platform followed by bioinformatics and statistical analyses. Results: The correlation between oral hygiene status and caries history varied from r2=0.887 to r2=0.999 in the geographical groups, suggesting oral hygiene status a strong association between caries history. Twenty taxonomic groups were found in all subjects and constituted 93.6%-96.5% of the microbiome. Of these, genus Veillonella and Prevotella showed significant differences in their proportions between the geographical groups (P
Databáze: OpenAIRE